Comparative study of poly tannic acid functionalized magnetic particles before and after modification for immobilized penicillin G acylase

doi:10.1080/09205063.2021.2021352

	Comparative study of poly tannic acid functionalized magnetic particles before and after modification for immobilized penicillin G acylase
	Tu, Hongyi 1,2; Gao, Kaikai 1,2; Zhang, Boyuan 1,2; Chen, Zhenbin 1,2; Wang, Pingbo 1,2; Li, Zhizhong 3
刊名	JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
	2021
卷号	33 期号:7 页码:823-846
关键词	Fe3O4 nanoparticles poly tannic acid physical adsorption glutaraldehyde covalent immobilization
ISSN号	0920-5063
DOI	10.1080/09205063.2021.2021352
英文摘要	In this work, Fe3O4 nanoparticles (NPs) was synthesized by inverting microemulsion method. After that, based on the physical and chemical properties of tannic acid (TA), poly tannic acid (PTA) was coated on Fe3O4 NPs surface. Fe3O4 NPs coated with PTA, on the one hand, was used to immobilize Penicillin G acylase (PGA) by physical adsorption. On the other hand, it was modified by glutaraldehyde (GA). GA grafting rate (G(r-GA)) was optimized, and the G(r-GA) was 30.0% under the optimum conditions. Then, through the Schiff base reaction between the glutaraldehyde group and PGA amino group, this covalent immobilization of PGA was further realized under mild conditions. Finally, the structures of every stage of magnetic composites were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), vibration magnetometer (VSM) and transmission electron microscopy (TEM), respectively. The results indicated that the enzyme activity (EA), enzyme activity recovery (EAR) and maximum load (ELC) of the immobilized PGA were 26843 U/g, 80.2% and 125 mg/g, respectively. Compared to the physical immobilization of PGA by only coating PTA nanoparticles, further modified nanoparticles by GA showed higher catalytic stability, reusability and storage stability.
WOS研究方向	Engineering ; Materials Science ; Polymer Science
语种	英语
出版者	TAYLOR & FRANCIS LTD
WOS记录号	WOS:000736830400001
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/154933]
专题	材料科学与工程学院
作者单位	1.Lanzhou Univ Technol, Sch Mat Sci & Engn, Lanzhou 730050, Peoples R China; 2.Lanzhou Univ Technol, State Key Lab Adv Progressing & Recycling Nonferr, Lanzhou, Peoples R China; 3.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou, Peoples R China
推荐引用方式 GB/T 7714	Tu, Hongyi,Gao, Kaikai,Zhang, Boyuan,et al. Comparative study of poly tannic acid functionalized magnetic particles before and after modification for immobilized penicillin G acylase[J]. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION,2021,33(7):823-846.
APA	Tu, Hongyi,Gao, Kaikai,Zhang, Boyuan,Chen, Zhenbin,Wang, Pingbo,&Li, Zhizhong.(2021).Comparative study of poly tannic acid functionalized magnetic particles before and after modification for immobilized penicillin G acylase.JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION,33(7),823-846.
MLA	Tu, Hongyi,et al."Comparative study of poly tannic acid functionalized magnetic particles before and after modification for immobilized penicillin G acylase".JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION 33.7(2021):823-846.