Study of target spacing of thermo-sensitive carrier on the activity recovery of immobilized penicillin G acylase

doi:10.1016/j.colsurfb.2019.03.064

	Study of target spacing of thermo-sensitive carrier on the activity recovery of immobilized penicillin G acylase
	Li, Ke 1,2; Shan, Guolei 1,2; Ma, Xiaobing 1,2; Zhang, Xinyu 1,2; Chen, Zhenbin 1,2; Tang, Zhenghua 3,4; Liu, Zhen 5
刊名	COLLOIDS AND SURFACES B-BIOINTERFACES
	2019-07
卷号	179 页码:153-160
关键词	Reactivity ratio Penicillin G acylase Carrier Activity recovery rate Target spacing
ISSN号	0927-7765
DOI	10.1016/j.colsurfb.2019.03.064
英文摘要	The immobilized penicillin G acylase (PGA) is an important industrial catalyst, the activity recovery rate of it directly affects enterprise efficiency. How to improve the enzyme activity recovery rate has been a research focus in this field. Based on the above problems, this work further improved the activity recovery rate by adjusting the target spacing for the first time. Glycidyl methacrylate (GMA) was used as the immobilized target and methyl methacrylate (MMA) as the copolymer monomer. According to the copolymer composition equation of P(MMA-co-GMA), the thermo-sensitive copolymers, PDEA-b-PHEMA-b-P(MMA-co-GMA) with different target spacings, were synthesized rapidly and efficiently via reversible addition-fragmentation chain transfer (RAFT) polymerization method. The error range between the theoretical and actual values of MMA and GMA in the copolymers carrier was (0-4)%, which demonstrated that the reliability of using composition equation to accurately and quickly synthesize copolymers with specific spacing. Studies on the thereto-sensitive showed that the low critical solution temperature (LCST) of the copolymer carrier decreased with the increase of hydrophobic monomer. Most importantly, the activity recovery rate increased with the increase of target spacing, and when the molar ratio of MMA to GMA in the copolymer was 8.75:1, the recovery of activity of immobilized PGA could be up to 63.50%, which was 21.70% higher than that of pure GMA. This work provided an important idea for improving the activity of immobilized PGA.
资助项目	National Natural Science Foundation of China[51563015]
WOS研究方向	Biophysics ; Chemistry ; Materials Science
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000471736600018
状态	已发表
内容类型	期刊论文
源URL	[http://119.78.100.223/handle/2XXMBERH/31775]
专题	材料科学与工程学院省部共建有色金属先进加工与再利用国家重点实验室
通讯作者	Chen, Zhenbin; Liu, Zhen
作者单位	1.Lanzhou Univ Technol, State Key Lab Gansu Adv Nonferrous Met Mat, Lanzhou 730050, Gansu, Peoples R China 2.Lanzhou Univ Technol, Sch Mat Sci & Engn, Lanzhou 730050, Gansu, Peoples R China 3.South China Univ Technol, Sch Environm & Energy, New Energy Res Inst, Guangzhou Key Lab Surface Chem Energy Mat,Guangzh, Guangzhou 5100067, Guangdong, Peoples R China 4.South China Univ Technol, Guangdong Prov Key Lab Atmospher Environm & Pollu, Guangdong Prov Engn & Technol Res Ctr Environm Ri, Sch Environm & Energy,Guangzhou Higher Educ Mega, Guangzhou 510006, Guangdong, Peoples R China 5.Frostburg State Univ, Dept Phys & Engn, Frostburg, MD 21532 USA
推荐引用方式 GB/T 7714	Li, Ke,Shan, Guolei,Ma, Xiaobing,et al. Study of target spacing of thermo-sensitive carrier on the activity recovery of immobilized penicillin G acylase[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2019,179:153-160.
APA	Li, Ke.,Shan, Guolei.,Ma, Xiaobing.,Zhang, Xinyu.,Chen, Zhenbin.,...&Liu, Zhen.(2019).Study of target spacing of thermo-sensitive carrier on the activity recovery of immobilized penicillin G acylase.COLLOIDS AND SURFACES B-BIOINTERFACES,179,153-160.
MLA	Li, Ke,et al."Study of target spacing of thermo-sensitive carrier on the activity recovery of immobilized penicillin G acylase".COLLOIDS AND SURFACES B-BIOINTERFACES 179(2019):153-160.