Biochar induced inhibitory effects on intracellular and extracellular antibiotic resistance genes in anaerobic digestion of swine manure

doi:10.1016/j.envres.2022.113530

CORC > 兰州理工大学 > 兰州理工大学

	Biochar induced inhibitory effects on intracellular and extracellular antibiotic resistance genes in anaerobic digestion of swine manure
	Yang, Shuo 2; Wen, Qinxue 2; Chen, Zhiqiang 1,2,3
刊名	ENVIRONMENTAL RESEARCH
	2022-09-01
卷号	212
关键词	Intracellular resistance genes Extracellular resistance genes Anaerobic digestion Biochar Antioxidant system Swine manure
ISSN号	0013-9351
DOI	10.1016/j.envres.2022.113530
英文摘要	Distribution of intracellular (iARGs) and extracellular ARGs (eARGs) in manure anaerobic digestion (AD) process coupled with two types of biochar (BC and BP) were investigated. And the effects of biochar on the conjugation transfer of ARGs were explored by deciphering the interaction of biochar with bacterial stress responses, physiological metabolism and antibiotic resistances. Results showed that AD process could effectively remove all the detected eARGs with efficiency of 47.4-98.2%. The modified biochar (BP) with larger specific surface area (SSA) was propitious to decrease the absolute copy number of extracellular resistance genes. AD process could effectively remove iARGs by inhibiting the growth of host bacteria. The results of structural equation models (SEM) indicated that biochar put indirect influences on the fate of ARGs (lambda =-0.23, P > 0.05). Analysis on oxidative stress levels, antioxidant capacity, DNA damage-induced response (SOS) response and energy gener-ation process demonstrated that biochar induced the oxidative stress response of microorganisms and enhanced the antioxidant capacity of bacteria. The elevated antioxidant capacity negatively affected SOS response, amplified cell membrane damage and further weakened the energy generation process, resulted in the inhibition of horizontal transfer of ARGs.
WOS研究方向	Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000810299500002
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/158884]
专题	兰州理工大学
作者单位	1.Lanzhou Univ Technol, Sch Civil Engn, Lanzhou 730070, Peoples R China; 2.Harbin Inst Technol, Sch Municipal & Environm Engn, State Key Lab Urban Water Resource & Environm, Harbin 150090, Peoples R China; 3.Harbin Inst Technol, Sch Environm, Harbin 150090, Peoples R China
推荐引用方式 GB/T 7714	Yang, Shuo,Wen, Qinxue,Chen, Zhiqiang. Biochar induced inhibitory effects on intracellular and extracellular antibiotic resistance genes in anaerobic digestion of swine manure[J]. ENVIRONMENTAL RESEARCH,2022,212.
APA	Yang, Shuo,Wen, Qinxue,&Chen, Zhiqiang.(2022).Biochar induced inhibitory effects on intracellular and extracellular antibiotic resistance genes in anaerobic digestion of swine manure.ENVIRONMENTAL RESEARCH,212.
MLA	Yang, Shuo,et al."Biochar induced inhibitory effects on intracellular and extracellular antibiotic resistance genes in anaerobic digestion of swine manure".ENVIRONMENTAL RESEARCH 212(2022).