Response characteristics of the membrane integrity and physiological activities of the mutant strain Y217 under exogenous butanol stress

doi:10.1007/s00253-021-11174-5

CORC > 兰州理工大学 > 兰州理工大学

	Response characteristics of the membrane integrity and physiological activities of the mutant strain Y217 under exogenous butanol stress
	Gao, Yue 2,5; Zhou, Xiang 2,5; Zhang, Miao-Miao 1,2,5; Liu, Ya-Jun 2,3; Guo, Xiao-Peng 4; Lei, Cai-Rong 2,5; Li, Wen-Jian 1,2,5; Lu, Dong 1,2,5
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2021-02
卷号	105 期号:6 页码:-
关键词	Binding sites Cell adhesion Genes Ion beams Ion bombardment Monounsaturated fatty acids Physiological models Proteins Saturated fatty acids Scanning electron microscopy Toxicity ATP binding cassette transporters Clostridium acetobutylicum Fluorescence intensities Membrane permeability Phosphotransferase system Physiological activity Response characteristic Transmembrane transport
ISSN号	0175-7598
DOI	10.1007/s00253-021-11174-5
英文摘要	Butanol inhibits bacterial activity by destroying the cell membrane of Clostridium acetobutylicum strains and altering functionality. Butanol toxicity also results in destruction of the phosphoenolpyruvate-carbohydrate phosphotransferase system (PTS), thereby preventing glucose transport and phosphorylation and inhibiting transmembrane transport and assimilation of sugars, amino acids, and other nutrients. In this study, based on the addition of exogenous butanol, the tangible macro indicators of changes in the carbon ion beam irradiation-mutant Y217 morphology were observed using scanning electron microscopy (SEM). The mutant has lower microbial adhesion to hydrocarbon (MATH) value than C. acetobutylicum ATCC 824 strain. FDA fluorescence intensity and conductivity studies demonstrated the intrinsically low membrane permeability of the mutant membrane, with membrane potential remaining relatively stable. Monounsaturated FAs (MUFAs) accounted for 35.17% of the mutant membrane, and the saturated fatty acids (SFA)/unsaturated fatty acids (UFA) ratio in the mutant cell membrane was 1.65. In addition, we conducted DNA-level analysis of the mutant strain Y217. Expectedly, through screening, we found gene mutant sites encoding membrane-related functions in the mutant, including ATP-binding cassette (ABC) transporter-related genes, predicted membrane proteins, and the PTS transport system. It is noteworthy that an unreported predicted membrane protein (CAC 3309) may be related to changes in mutant cell membrane properties.
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	Springer Science and Business Media Deutschland GmbH
WOS记录号	WOS:000619755300001
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/147280]
专题	兰州理工大学
作者单位	1.Gansu Key Lab Microbial Resources Exploitat & App, Lanzhou 730070, Peoples R China; 2.Chinese Acad Sci, Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China; 4.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou, Peoples R China 5.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China;
推荐引用方式 GB/T 7714	Gao, Yue,Zhou, Xiang,Zhang, Miao-Miao,et al. Response characteristics of the membrane integrity and physiological activities of the mutant strain Y217 under exogenous butanol stress[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2021,105(6):-.
APA	Gao, Yue.,Zhou, Xiang.,Zhang, Miao-Miao.,Liu, Ya-Jun.,Guo, Xiao-Peng.,...&Lu, Dong.(2021).Response characteristics of the membrane integrity and physiological activities of the mutant strain Y217 under exogenous butanol stress.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,105(6),-.
MLA	Gao, Yue,et al."Response characteristics of the membrane integrity and physiological activities of the mutant strain Y217 under exogenous butanol stress".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 105.6(2021):-.