Synthesis and protein immobilization of monodisperse magnetic spheres with multifunctional groups

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	Synthesis and protein immobilization of monodisperse magnetic spheres with multifunctional groups
	Yang, CL ; Guan, YP ; Xing, JM ; Jia, GH ; Liu, HZ
刊名	REACTIVE & FUNCTIONAL POLYMERS
	2006-02-01
卷号	66 期号:2 页码:267-273
关键词	characterization superparamagnetic monodisperse spheres multifunctional groups spraying suspension polymerization protein immobilization
ISSN号	1381-5148
其他题名	React. Funct. Polym.
中文摘要	Monodisperse magnetic spheres with multifunctional groups were synthesized by spraying suspension polymerization. The effects of stabilizer type and its concentration on the monodispersity of magnetic spheres were investigated. The results showed that polyvinyl alcohol (PVA) was satisfactory as a stabilizer in this spraying suspension polymerization, and the magnetic spheres showed good monodispersity when the concentration of PVA was increased to above 20 wt%. It was also found that the monodispersity of magnetic spheres could be improved with increase of the amount of initiator. Scanning electron microscopy and vibrating sample magnetometer verified that the magnetic spheres obtained were monodisperse with small size (about 9.8 mu m) and displayed superparamagnetic characteristics. Data of Fourier transform infrared (FT-IR) spectroscopy and surface chemical reaction method indicated that the extensive epoxy groups existed on the surface of magnetic spheres. Bovine serum albumin (BSA) was covalently immobilized onto the glutaraldehyde treated and untreated magnetic spheres, respectively. The results showed that the maximum BSA immobilization capacity onto the magnetic spheres was about 57 mg/g. (c) 2005 Elsevier B.V. All rights reserved.
英文摘要	Monodisperse magnetic spheres with multifunctional groups were synthesized by spraying suspension polymerization. The effects of stabilizer type and its concentration on the monodispersity of magnetic spheres were investigated. The results showed that polyvinyl alcohol (PVA) was satisfactory as a stabilizer in this spraying suspension polymerization, and the magnetic spheres showed good monodispersity when the concentration of PVA was increased to above 20 wt%. It was also found that the monodispersity of magnetic spheres could be improved with increase of the amount of initiator. Scanning electron microscopy and vibrating sample magnetometer verified that the magnetic spheres obtained were monodisperse with small size (about 9.8 mu m) and displayed superparamagnetic characteristics. Data of Fourier transform infrared (FT-IR) spectroscopy and surface chemical reaction method indicated that the extensive epoxy groups existed on the surface of magnetic spheres. Bovine serum albumin (BSA) was covalently immobilized onto the glutaraldehyde treated and untreated magnetic spheres, respectively. The results showed that the maximum BSA immobilization capacity onto the magnetic spheres was about 57 mg/g. (c) 2005 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Physical Sciences ; Technology
类目[WOS]	Chemistry, Applied ; Engineering, Chemical ; Polymer Science
研究领域[WOS]	Chemistry ; Engineering ; Polymer Science
关键词[WOS]	METHACRYLATE) MICROSPHERES ; DISPERSION POLYMERIZATION ; SUSPENSION POLYMERIZATION ; ADSORPTION ; PARTICLES ; SUPPORTS ; BEHAVIOR
收录类别	SCI
原文出处	://WOS:000235385900006
语种	英语
WOS记录号	WOS:000235385900006
公开日期	2013-10-24
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/4094]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Lab Separat Sci & Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Yang, CL,Guan, YP,Xing, JM,et al. Synthesis and protein immobilization of monodisperse magnetic spheres with multifunctional groups[J]. REACTIVE & FUNCTIONAL POLYMERS,2006,66(2):267-273.
APA	Yang, CL,Guan, YP,Xing, JM,Jia, GH,&Liu, HZ.(2006).Synthesis and protein immobilization of monodisperse magnetic spheres with multifunctional groups.REACTIVE & FUNCTIONAL POLYMERS,66(2),267-273.
MLA	Yang, CL,et al."Synthesis and protein immobilization of monodisperse magnetic spheres with multifunctional groups".REACTIVE & FUNCTIONAL POLYMERS 66.2(2006):267-273.