Effect of amphiphilic block copolymer pluronic P103 on the conformation of bovine serum albumin

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	Effect of amphiphilic block copolymer pluronic P103 on the conformation of bovine serum albumin
	Wang Jing ; Guo Chen ; Liang Xiangfeng ; Zheng Lili ; Chen Shu ; Ma Junhe ; Liu Huizhou
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2006-12-01
卷号	34 期号:12 页码:1711-1714
关键词	block copolymer bovine serum albumin Fourier trastonit infrared Fourier transform-Raman
ISSN号	0253-3820
其他题名	Chin. J. Anal. Chem.
中文摘要	Fourier transform infrared and Fournier transform -Raman spectroscopy were applied to investigate the interaction between Poly( ethylene oxide) -poly( propylene oxide) -poly( ethylene oxide) ( PEO-PPO-PEO) block copolymer (Pluronic P103) and bovine serum albumin (BSA). Microenvironment of some amino acids and the conformation of S-S bond in BSA were influenced by Pluronic P103, when Pluronic P103 was added. Pluronic P103 also could change some alpha-helixes into beta-sheets. The secondary structure of BSA showed little change at lower P103 concentration when the concentration of P103 is not lower than 16 g/L. alpha-helixes decreased from 45. 9% to 40%, and beta-sheets increased from 6. 1% to 17%, in the meantime the random structure showed a little decrease.
英文摘要	Fourier transform infrared and Fournier transform -Raman spectroscopy were applied to investigate the interaction between Poly( ethylene oxide) -poly( propylene oxide) -poly( ethylene oxide) ( PEO-PPO-PEO) block copolymer (Pluronic P103) and bovine serum albumin (BSA). Microenvironment of some amino acids and the conformation of S-S bond in BSA were influenced by Pluronic P103, when Pluronic P103 was added. Pluronic P103 also could change some alpha-helixes into beta-sheets. The secondary structure of BSA showed little change at lower P103 concentration when the concentration of P103 is not lower than 16 g/L. alpha-helixes decreased from 45. 9% to 40%, and beta-sheets increased from 6. 1% to 17%, in the meantime the random structure showed a little decrease.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	TRIBLOCK COPOLYMERS ; SPECTROSCOPY ; RAMAN
收录类别	SCI
原文出处	://WOS:000243388100010
语种	英语
WOS记录号	WOS:000243388100010
公开日期	2013-10-24
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/3954]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Young Scientist Lab Separat Sci & Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang Jing,Guo Chen,Liang Xiangfeng,et al. Effect of amphiphilic block copolymer pluronic P103 on the conformation of bovine serum albumin[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(12):1711-1714.
APA	Wang Jing.,Guo Chen.,Liang Xiangfeng.,Zheng Lili.,Chen Shu.,...&Liu Huizhou.(2006).Effect of amphiphilic block copolymer pluronic P103 on the conformation of bovine serum albumin.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34(12),1711-1714.
MLA	Wang Jing,et al."Effect of amphiphilic block copolymer pluronic P103 on the conformation of bovine serum albumin".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34.12(2006):1711-1714.