Application of a pH Feedback-Controlled Substrate Feeding Method in Lactic Acid Production

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	Application of a pH Feedback-Controlled Substrate Feeding Method in Lactic Acid Production
	Zhang, Yong 1,2; Cong, Wei 1,2; Shi, ShaoYuan 1,2
刊名	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
	2010-12-01
卷号	162 期号:8 页码:2149-2156
关键词	Lactic acid Fed-batch Lactobacillus lactis-11 Ammonium hydroxide pH feedback
ISSN号	0273-2289
通讯作者	Cong, W
英文摘要	Substrate concentration in lactic acid fermentation broth could not be controlled well by traditional feeding methods, including constant, intermittent, and exponential feeding methods, in fed-batch experiments. A simple feedback feeding method based on pH was proposed to control pH and substrate concentration synchronously to enhance lactic acid production in fed-batch culture. As the linear relationship between the consumption amounts of alkali and that of substrate was concluded during lactic acid fermentation, the alkali and substrate in the feeding broth were mixed together proportionally. Thus, the concentration of substrate could be controlled through the adjustment of pH automatically. In the fed-batch lactic acid fermentation with Lactobacillus lactis-11 by this method, the residual glucose concentration in fermentation broth was controlled between 4.1 and 4.9 g L-1, and the highest concentration of lactic acid, maximum cell dry weight, volumetric productivity of lactic acid, and yield were 96.3 g L-1, 4.7 g L-1, 1.9 g L-1 h(-1) and 0.99 g lactic acid per gram of glucose, respectively, compared to 82.7 g L-1, 3.31 g L-1 1.7 g L-1 h(-1) and 0.92 g lactic acid per gram of glucose in batch culture. This feeding method was simple and easily operated and could be feasible for industrial lactic acid production in the future.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]	LACTOBACILLUS-AMYLOPHILUS GV6 ; FED-BATCH CULTURE ; RHIZOPUS-ORYZAE ; SIMULTANEOUS SACCHARIFICATION ; DIRECT FERMENTATION ; AMMONIUM LACTATE ; SUBSP-LACTIS ; STARCH ; BROTH
收录类别	SCI
语种	英语
WOS记录号	WOS:000284295000003
公开日期	2013-11-11
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/5411]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yong,Cong, Wei,Shi, ShaoYuan. Application of a pH Feedback-Controlled Substrate Feeding Method in Lactic Acid Production[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2010,162(8):2149-2156.
APA	Zhang, Yong,Cong, Wei,&Shi, ShaoYuan.(2010).Application of a pH Feedback-Controlled Substrate Feeding Method in Lactic Acid Production.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,162(8),2149-2156.
MLA	Zhang, Yong,et al."Application of a pH Feedback-Controlled Substrate Feeding Method in Lactic Acid Production".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 162.8(2010):2149-2156.