Determination of the nucleosides and nucleobases in Tuber samples by dispersive solid-phase extraction combined with liquid chromatography-mass spectrometry

CORC > 过程工程研究所 > 中国科学院过程工程研究所 > 生化工程国家重点实验室

	Determination of the nucleosides and nucleobases in Tuber samples by dispersive solid-phase extraction combined with liquid chromatography-mass spectrometry
	Liu, Ping 1; Li, Yuan-Yuan 1,4; Li, Hong-Mei 1; Wan, Duan-Ji 1; Tang, Ya-Jie 1,2,3
刊名	ANALYTICA CHIMICA ACTA
	2011-02-21
卷号	687 期号:2 页码:159-167
关键词	Truffle Nucleosides Nucleobases Protein removal Dispersive solid-phase extraction Liquid chromatography-mass spectrometry (LC-MS)
ISSN号	0003-2670
通讯作者	Tang, YJ
英文摘要	A simple, fast and inexpensive method based on dispersive solid phase extraction (DSPE) combined with LC-MS was developed for simultaneous determination of 7 nucleosides and nucleobases (i.e., adenine, hypoxanthine, uridine, adenosine, guanine, guanosine, and inosine) in Tuber fruiting-bodies and fermentation mycelia. The DSPE procedure was firstly introduced to remove the protein interference from sample solutions, and D3520 macroporous resin was chosen as the DSPE sorbent because of its high removal capability on protein interferences, but low adsorption rate on analytes. Besides, key parameters on DSPE procedure (i.e., macroporous resin type, macroporous resin amount, methanol concentration, and vortex time) were optimized, and the protein removal efficacy could achieve about 95% after the process optimization. Though the method validation test, the DSPE-LC-MS method was confirmed to be precise, accurate and sensitive, and the column blinding problem was solved successfully. By using this established method, the total amount of nucleosides and nucleobases in the fermentation mycelia was determined to range from 4881.5 to 12,592.9 mu g g(-1), which was about 2-25 times higher than the fruiting-bodies (from 498.1 to 2274.1 mu g g(-1)). The formulation of nucleosides and nucleobases in the fermentation mycelia maintained relatively constant, while the formulation in Tuber fruiting-bodies varied significantly with their species. Hierarchical cluster analysis (HCA) showed the formulation similarity of nucleosides and nucleobases between Tuber fermentation mycelia and the fruiting-bodies of Tuber indicum and Tuber himalayense. From the viewpoint of nucleosides and nucleobases, this work confirms the potentiality of Tuber fermentation mycelia as the alternative resource for its fruiting-bodies. (C) 2010 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	BETA-LACTAM ANTIBIOTICS ; MACROPOROUS RESINS ; POLYSACCHARIDES PRODUCTION ; CAPILLARY-ELECTROPHORESIS ; PESTICIDE-RESIDUES ; CULTURED CORDYCEPS ; CELL-GROWTH ; BOVINE-MILK ; FERMENTATION ; SINENSE
收录类别	SCI
语种	英语
WOS记录号	WOS:000287564700008
公开日期	2013-11-07
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/5087]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Hubei Univ Technol, Coll Bioengn, Hubei Prov Key Lab Ind Microbiol, Minist Educ,Key Lab Fermentat Engn, Wuhan 430068, Peoples R China 2.Chinese Acad Sci, Shanghai Adv Res Inst, Lab Biorefinery Engn, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 4.City Univ Hong Kong, Dept Biol & Chem, Res Grp Bioact Prod, Kowloon, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Liu, Ping,Li, Yuan-Yuan,Li, Hong-Mei,et al. Determination of the nucleosides and nucleobases in Tuber samples by dispersive solid-phase extraction combined with liquid chromatography-mass spectrometry[J]. ANALYTICA CHIMICA ACTA,2011,687(2):159-167.
APA	Liu, Ping,Li, Yuan-Yuan,Li, Hong-Mei,Wan, Duan-Ji,&Tang, Ya-Jie.(2011).Determination of the nucleosides and nucleobases in Tuber samples by dispersive solid-phase extraction combined with liquid chromatography-mass spectrometry.ANALYTICA CHIMICA ACTA,687(2),159-167.
MLA	Liu, Ping,et al."Determination of the nucleosides and nucleobases in Tuber samples by dispersive solid-phase extraction combined with liquid chromatography-mass spectrometry".ANALYTICA CHIMICA ACTA 687.2(2011):159-167.