In Vitro Reduction of Hexavalent Chromium by Cytoplasmic Fractions of Pannonibacter phragmitetus LSSE-09 under Aerobic and Anaerobic Conditions

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	In Vitro Reduction of Hexavalent Chromium by Cytoplasmic Fractions of Pannonibacter phragmitetus LSSE-09 under Aerobic and Anaerobic Conditions
	Xu, Lin 1,2,4; Luo, Mingfang 3; Jiang, Chengying 3; Wei, Xuetuan 1,2,4; Kong, Peng 1,2,4; Liang, Xiangfeng 1,2; Zhao, Junmei 1,2; Yang, Liangrong 1,2; Liu, Huizhou 1,2
刊名	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
	2012-02-01
卷号	166 期号:4 页码:933-941
关键词	Pannonibacter phragmitetus LSSE-09 Cr(VI) reduction Chromate reductase Cytoplasmic fractions
ISSN号	0273-2289
通讯作者	Liu, HZ
英文摘要	Hexavalent chromate reductase was characterized and was found to be localized in the cytoplasmic fraction of a chromium-resistant bacterium Pannonibacter phragmitetus LSSE-09. The Cr(VI) reductase activity of cell-free extract (S-12) was significantly improved by external electron donors, such as NADH, glucose, acetate, formate, citrate, pyruvate, and lactate. The reductase activity was optimal at pH 7.0 with NADH as the electron donor. The aerobic and anaerobic Cr(VI)-reduction enhanced by 0.1 mM NADH were respectively 3.5 and 3.4 times as high as that without adding NADH. The Cr(VI) reductase activity was inhibited by Mn2+, Cd2+, Fe3+, and Hg2+, whereas Cu2+ enhanced the chromate reductase activity by 29% aerobically and 33% anaerobically. The aerobic and anaerobic specific Michaelis-Menten constant K (m) of S-12 fraction was estimated to be 64.95 and 47.65 mu mol L-1, respectively. The soluble S-150 fractions showed similar activity to S-12 and could reduce 39.7% and 53.4% of Cr(VI) after 1 h of incubation aerobically and anaerobically while the periplasmic contents showed no obvious reduction activity, suggesting an effective enzymatic mechanism of Cr(VI) reduction in the cytoplasmic fractions of the bacterium. Results suggest that the enzymatic reduction of Cr(VI) could be useful for Cr(VI) detoxification in wastewater.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]	SULFATE-REDUCING BACTERIA ; CELL-FREE-EXTRACT ; CHROMATE REDUCTION ; ESCHERICHIA-COLI ; PSEUDOMONAS-PUTIDA ; ENTEROBACTER-CLOACAE ; ENZYMATIC REDUCTION ; BACILLUS SP ; CR(VI) ; HYDROGENASES
收录类别	SCI
语种	英语
WOS记录号	WOS:000300296700010
公开日期	2013-10-26
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/4295]
专题	过程工程研究所_湿法冶金清洁生产技术国家工程实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xu, Lin,Luo, Mingfang,Jiang, Chengying,et al. In Vitro Reduction of Hexavalent Chromium by Cytoplasmic Fractions of Pannonibacter phragmitetus LSSE-09 under Aerobic and Anaerobic Conditions[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2012,166(4):933-941.
APA	Xu, Lin.,Luo, Mingfang.,Jiang, Chengying.,Wei, Xuetuan.,Kong, Peng.,...&Liu, Huizhou.(2012).In Vitro Reduction of Hexavalent Chromium by Cytoplasmic Fractions of Pannonibacter phragmitetus LSSE-09 under Aerobic and Anaerobic Conditions.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,166(4),933-941.
MLA	Xu, Lin,et al."In Vitro Reduction of Hexavalent Chromium by Cytoplasmic Fractions of Pannonibacter phragmitetus LSSE-09 under Aerobic and Anaerobic Conditions".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 166.4(2012):933-941.