Tlx3 Controls Cholinergic Transmitter and Peptide Phenotypes in a Subset of Prenatal Sympathetic Neurons

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	Tlx3 Controls Cholinergic Transmitter and Peptide Phenotypes in a Subset of Prenatal Sympathetic Neurons
	Huang, TW ; Hu, J ; Wang, B ; Nie, YZ ; Geng, JL ; Cheng, LP
刊名	JOURNAL OF NEUROSCIENCE
	2013
卷号	33 期号:26 页码:10667-10675
关键词	VASOACTIVE-INTESTINAL-PEPTIDE SWEAT GLAND INNERVATION NEUROTRANSMITTER PHENOTYPE NERVOUS-SYSTEM DEVELOPMENTAL EXPRESSION TRANSCRIPTION FACTOR STELLATE GANGLION NEURAL CREST LIFR-BETA DIFFERENTIATION
ISSN号	0270-6474
通讯作者	Cheng, LP (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,lpcheng@ion.ac.cn
英文摘要	The embryonic sympathetic nervous system consists of predominantly noradrenergic neurons and a very small population of cholinergic neurons. Postnatal development further allows target-dependent switch of a subset of noradrenergic neurons into cholinergic phenotype. How embryonic cholinergic neurons are specified at the prenatal stages remains largely unknown. In this study, we found that the expression of transcription factor Tlx3 was progressively restricted to a small population of embryonic sympathetic neurons in mice. Immunostaining for vesicular acetylcholine transporter (VAChT) showed that Tlx3 was highly expressed in cholinergic neurons at the late embryonic stage E18.5. Deletion of Tlx3 resulted in the loss of Vacht expression at E18.5 but not E12.5. By contrast, Tlx3 was required for expression of the cholinergic peptide vasoactive intestinal polypeptide (VIP), and somatostatin (SOM) at both E12.5 and E18.5. Furthermore, we found that, at E18.5 these putative cholinergic neurons expressed glial cell line-derived neurotrophic factor family coreceptor Ret but not tyrosine hydroxylase (Ret(+)/TH-). Deletion of Tlx3 also resulted in disappearance of high-level Ret expression. Last, unlike Tlx3, Ret was required for the expression of VIP and SOM at E18.5 but not E12.5. Together, these results indicate that transcription factor Tlx3 is required for the acquisition of cholinergic phenotype at the late embryonic stage as well as the expression and maintenance of cholinergic peptides VIP and SOM throughout prenatal development of mouse sympathetic neurons.
学科主题	Neurosciences & Neurology
收录类别	SCI
资助信息	Chinese Academy of Sciences [XDA01020306]; The Ministry of Science and Technology of China [2011 CBA00400]
语种	英语
WOS记录号	WOS:000320928900012
公开日期	2013-11-06
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/2570]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Huang, TW,Hu, J,Wang, B,et al. Tlx3 Controls Cholinergic Transmitter and Peptide Phenotypes in a Subset of Prenatal Sympathetic Neurons[J]. JOURNAL OF NEUROSCIENCE,2013,33(26):10667-10675.
APA	Huang, TW,Hu, J,Wang, B,Nie, YZ,Geng, JL,&Cheng, LP.(2013).Tlx3 Controls Cholinergic Transmitter and Peptide Phenotypes in a Subset of Prenatal Sympathetic Neurons.JOURNAL OF NEUROSCIENCE,33(26),10667-10675.
MLA	Huang, TW,et al."Tlx3 Controls Cholinergic Transmitter and Peptide Phenotypes in a Subset of Prenatal Sympathetic Neurons".JOURNAL OF NEUROSCIENCE 33.26(2013):10667-10675.