Quantitative proteomic strategies for the identification of microRNA targets

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物分子与细胞生物学研究中心 > 期刊论文

	Quantitative proteomic strategies for the identification of microRNA targets
	Li, Chongyang 1,2; Xiong, Qian 1; Zhang, Jia 3; Ge, Feng 1; Bi, Li-Jun 3
刊名	EXPERT REVIEW OF PROTEOMICS
	2012-10-01
卷号	9 期号:5 页码:549-559
关键词	2D difference gel electrophoresis isobaric tags for relative and absolute quantification isotope-coded affinity tags microRNAs pulsed stable-isotope labeling by amino acids in cell culture quantitative proteomics stable-isotope labeling by amino acids in cell culture
ISSN号	1478-9450
通讯作者	Ge, F (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
中文摘要	MicroRNAs (miRNAs) are small noncoding RNAs, approximately 22 nucleotides in length, found in diverse organisms. They have emerged in recent years as key regulators of a broad spectrum of cellular functions. miRNAs regulate biological processes by inducing translational inhibition and degradation of their target mRNAs through base pairing to partially or fully complementary sites. In the field of miRNA research, the identification of the targets of individual miRNAs is of utmost importance. Our understanding of the molecular mechanisms by which individual miRNAs modulate cellular functions will remain incomplete until a full set of miRNA targets is identified and validated. Since a miRNA may regulate many of its targets at the translational level without affecting mRNA abundance, proteomic methods are best suited for revealing the full spectrum of miRNA targets. Quantitative proteomics is emerging as a powerful toolbox for identifying miRNA targets and for quantifying the contribution of translational repression by miRNAs. In this review, the authors summarize the quantitative proteomic approaches that have been employed for identification of miRNA targets and discuss current challenges as well as possible ways of overcoming them.
英文摘要	MicroRNAs (miRNAs) are small noncoding RNAs, approximately 22 nucleotides in length, found in diverse organisms. They have emerged in recent years as key regulators of a broad spectrum of cellular functions. miRNAs regulate biological processes by inducing translational inhibition and degradation of their target mRNAs through base pairing to partially or fully complementary sites. In the field of miRNA research, the identification of the targets of individual miRNAs is of utmost importance. Our understanding of the molecular mechanisms by which individual miRNAs modulate cellular functions will remain incomplete until a full set of miRNA targets is identified and validated. Since a miRNA may regulate many of its targets at the translational level without affecting mRNA abundance, proteomic methods are best suited for revealing the full spectrum of miRNA targets. Quantitative proteomics is emerging as a powerful toolbox for identifying miRNA targets and for quantifying the contribution of translational repression by miRNAs. In this review, the authors summarize the quantitative proteomic approaches that have been employed for identification of miRNA targets and discuss current challenges as well as possible ways of overcoming them.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	LONG NONCODING RNAS ; GEL-ELECTROPHORESIS ; CANCER ; SILAC ; BIOGENESIS ; PROTEINS ; ELEGANS ; MIR-21 ; CELLS ; TRANSLATION
收录类别	SCI
资助信息	National Basic Research Program of China (973 Program) [2012CB518700]; Hundred Talents Program of the Chinese Academy of Sciences
语种	英语
WOS记录号	WOS:000312604600016
公开日期	2013-10-31
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19346]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Inst Biophys, Key Lab Noncoding RNA, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Li, Chongyang,Xiong, Qian,Zhang, Jia,et al. Quantitative proteomic strategies for the identification of microRNA targets[J]. EXPERT REVIEW OF PROTEOMICS,2012,9(5):549-559.
APA	Li, Chongyang,Xiong, Qian,Zhang, Jia,Ge, Feng,&Bi, Li-Jun.(2012).Quantitative proteomic strategies for the identification of microRNA targets.EXPERT REVIEW OF PROTEOMICS,9(5),549-559.
MLA	Li, Chongyang,et al."Quantitative proteomic strategies for the identification of microRNA targets".EXPERT REVIEW OF PROTEOMICS 9.5(2012):549-559.