Effects of acute exposure to polybrominated diphenyl ethers on retinoid signaling in zebrafish larvae | |
Xu, Tao1; Chen, Lianguo2; Hu, Chenyan2; Zhou, Bingsheng2 | |
刊名 | ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY |
2013 | |
卷号 | 35期号:1页码:13-20 |
关键词 | Polybrominated diphenyl ethers Retinoid signaling Retinoic acid Rhodopsin Zebrafish embryos |
ISSN号 | 1382-6689 |
通讯作者 | Chen, LG (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China. |
中文摘要 | The objectives of the present study were to investigate the effects of acute exposure to PBDEs on retinoid signaling in fish. Zebrafish embryos (2 h post-fertilization, hpf) were exposed to DE-71 (0, 31.0, 68.7, and 227.6 mu g/L) until 120 hpf. Retinoid profiles showed the content of retinal and retinoic acid was reduced significantly. While a significant up-regulation was observed in the transcription of retinal dehydrogenase (raldh2), the transcription of retinol binding protein (rbp1a), retinol dehydrogenase (rdh1), cellular retinoic acid binding protein.(crabp1a and crabp2a) and retinoic acid receptor subunit (raraa) were down-regulated significantly, indicating disruption of retinoid signaling. However, the transcriptions of five opsin genes (zfrho, zfuv, zfred, zfblue, and zfgr1) were up-regulated. Furthermore, whole mount immunostaining and western blotting demonstrated increased rhodopsin protein expression in the exposure groups. Overall, the results indicated that acute exposure to PBDEs could disturb retinoid signaling and may impact on eye development of zebrafish larvae. (C) 2012 Elsevier B.V. All rights reserved. |
英文摘要 | The objectives of the present study were to investigate the effects of acute exposure to PBDEs on retinoid signaling in fish. Zebrafish embryos (2 h post-fertilization, hpf) were exposed to DE-71 (0, 31.0, 68.7, and 227.6 mu g/L) until 120 hpf. Retinoid profiles showed the content of retinal and retinoic acid was reduced significantly. While a significant up-regulation was observed in the transcription of retinal dehydrogenase (raldh2), the transcription of retinol binding protein (rbp1a), retinol dehydrogenase (rdh1), cellular retinoic acid binding protein.(crabp1a and crabp2a) and retinoic acid receptor subunit (raraa) were down-regulated significantly, indicating disruption of retinoid signaling. However, the transcriptions of five opsin genes (zfrho, zfuv, zfred, zfblue, and zfgr1) were up-regulated. Furthermore, whole mount immunostaining and western blotting demonstrated increased rhodopsin protein expression in the exposure groups. Overall, the results indicated that acute exposure to PBDEs could disturb retinoid signaling and may impact on eye development of zebrafish larvae. (C) 2012 Elsevier B.V. All rights reserved. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Environmental Sciences ; Pharmacology & Pharmacy ; Toxicology |
研究领域[WOS] | Environmental Sciences & Ecology ; Pharmacology & Pharmacy ; Toxicology |
关键词[WOS] | BROMINATED FLAME RETARDANTS ; VITAMIN-A ; GENE-EXPRESSION ; MATERNAL TRANSFER ; PHOTORECEPTOR DEVELOPMENT ; ENVIRONMENTAL-POLLUTANTS ; EMBRYONIC-DEVELOPMENT ; THYROID-HORMONE ; DANIO-RERIO ; ACID |
收录类别 | SCI |
资助信息 | National Natural Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2012FB17] |
语种 | 英语 |
WOS记录号 | WOS:000314262300002 |
公开日期 | 2013-10-31 |
内容类型 | 期刊论文 |
源URL | [http://ir.ihb.ac.cn/handle/342005/19330] |
专题 | 水生生物研究所_水环境工程研究中心_期刊论文 |
作者单位 | 1.Huazhong Univ Sci & Technol, Puai Hosp, Wuhan 430033, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
推荐引用方式 GB/T 7714 | Xu, Tao,Chen, Lianguo,Hu, Chenyan,et al. Effects of acute exposure to polybrominated diphenyl ethers on retinoid signaling in zebrafish larvae[J]. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,2013,35(1):13-20. |
APA | Xu, Tao,Chen, Lianguo,Hu, Chenyan,&Zhou, Bingsheng.(2013).Effects of acute exposure to polybrominated diphenyl ethers on retinoid signaling in zebrafish larvae.ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,35(1),13-20. |
MLA | Xu, Tao,et al."Effects of acute exposure to polybrominated diphenyl ethers on retinoid signaling in zebrafish larvae".ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 35.1(2013):13-20. |
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