Biochemical characterization of caffeoyl coenzyme A 3-O-methyltransferase from wheat

doi:10.1007/s00425-015-2295-3

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	Biochemical characterization of caffeoyl coenzyme A 3-O-methyltransferase from wheat
	Ma, Qing-Hu ; Luo, Hao-Ran
刊名	PLANTA
	2015
卷号	242 期号:1 页码:113-122
关键词	Caffeoyl coenzyme A 3-O-methyltransferase (CCoAOMT) Lignin biosynthesis Lodging resistance Stem development Triticum
ISSN号	0032-0935
DOI	10.1007/s00425-015-2295-3
文献子类	Article
英文摘要	TaCCoAOMT1 is located in wheat chromosome 7A and highly expressed in stem and root. It is important for lignin biosynthesis, and associated with stem maturity but not lodging resistance. Caffeoyl coenzyme A 3-O-methyltransferases (CCoAOMTs) are one important class of enzymes to carry out the transfer of the methyl group from S-adenosylmethionine to the hydroxyl group, and play important roles in lignin and flavonoids biosynthesis. In the present study, sequences for CCoAOMT from the wheat genome were analyzed. One wheat CCoAOMT that belonged to bona fide subclade involved in lignin biosynthesis, namely TaCCoAOMT1, was obtained by the prokaryotic expression in E. coli. The three-dimensional structure prediction showed a highly similar structure of TaCCoAOMT1 with MsCCoAOMT. Recombinant TaCCoAOMT1 protein could only use caffeoyl CoA and 5-hydroxyferuloyl CoA as effective substrates and caffeoyl CoA as the best substrate. TaCCoAOMT1 had a narrow optimal pH and thermal stability. The TaCCoAOMT1 gene was highly expressed in wheat stem and root tissues, paralleled CCoAOMT enzyme activity. TaCCoAOMT1 mRNA abundance and enzyme activity increased linearly with stem maturity, but showed little difference between wheat lodging-resistant (H4546) and lodging-sensitive (C6001) cultivars in elongation, heading and milky stages. These data suggest that TaCCoAOMT1 is an important CCoAOMT for lignin biosynthesis that is critical for stem development, but not directly associated with lodging-resistant trait in wheat.
学科主题	Plant Sciences
电子版国际标准刊号	1432-2048
出版地	NEW YORK
WOS关键词	CELL-SUSPENSION CULTURES ; ADENOSYL-L-METHIONINE ; ACID/5-HYDROXYFERULIC ACID 3/5-O-METHYLTRANSFERASE ; CINNAMOYL-COA REDUCTASE ; O-METHYLTRANSFERASE ; LIGNIN BIOSYNTHESIS ; ARABIDOPSIS-THALIANA ; DOWN-REGULATION ; METHYLATION PATHWAY ; PHENYLPROPANOID METABOLISM
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000356516800008
资助机构	National Natural Science Foundation of China [31370336, 31170277] ; National Key Basic Research Program of China (973 program) ; Natural Science Foundation of Beijing [5122024] ; Innovation Project of Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25585]
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.[Ma, Qing-Hu
推荐引用方式 GB/T 7714	Ma, Qing-Hu,Luo, Hao-Ran. Biochemical characterization of caffeoyl coenzyme A 3-O-methyltransferase from wheat[J]. PLANTA,2015,242(1):113-122.
APA	Ma, Qing-Hu,&Luo, Hao-Ran.(2015).Biochemical characterization of caffeoyl coenzyme A 3-O-methyltransferase from wheat.PLANTA,242(1),113-122.
MLA	Ma, Qing-Hu,et al."Biochemical characterization of caffeoyl coenzyme A 3-O-methyltransferase from wheat".PLANTA 242.1(2015):113-122.