OsKinesin-13A Is an Active Microtubule Depolymerase Involved in Glume Length Regulation via Affecting Cell Elongation

doi:10.1038/srep09457

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	OsKinesin-13A Is an Active Microtubule Depolymerase Involved in Glume Length Regulation via Affecting Cell Elongation
	Deng, Zhu Yun ; Liu, Ling Tong 4; Li, Tang ; Yan, Song 1; Kuang, Bai Jian ; Huang, Shan Jin 3; Yan, Chang Jie 1; Wang, Tai
刊名	SCIENTIFIC REPORTS
	2015
卷号	5
ISSN号	2045-2322
DOI	10.1038/srep09457
文献子类	Article
英文摘要	Grain size is an important trait influencing both the yield and quality of rice and its major determinant is glume size. However, how glume size is regulated remains largely unknown. Here, we report the characterization of OsKinesin-13A, which regulates cell elongation and glume length in rice. The mutant of OsKinesin-13A, sar1, displayed length reduction in grains and other organs including internodes, leaves and roots. The grain phenotype in sar1 was directly caused by reduction in glume length, which in turn restricted caryopsis size. Histological results revealed that length decrease in sar1 organs resulted from abnormalities in cell elongation. The orientation of cellulose microfibrils was defective in sar1. Consistently, sar1 showed reduced transverse orientation of cortical microtubules. Further observations demonstrated that microtubule turnover was decreased in sar1. OsKinesin-13A was shown to be an active microtubule depolymerase and mainly distributed on vesicles derived from the Golgi apparatus and destined for the cell surface. Thus, our results suggest that OsKinesin-13A utilizes its microtubule depolymerization activity to promote microtubule turnover, which may not only influence transverse orientation of cortical microtubules but also facilitate vesicle transport from the Golgi apparatus to the cell surface, and thus affects cellulose microfibril orientation and cell elongation.
学科主题	Multidisciplinary Sciences
出版地	LONDON
WOS关键词	RICE GRAIN WIDTH ; GOLGI-APPARATUS ; PLASMA-MEMBRANE ; GENE ENCODES ; PROTEIN ; SIZE ; KINESIN ; YIELD ; GROWTH ; ORGANIZATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000351703700001
资助机构	National Natural Science Foundation of China [31000694] ; Chinese Ministry of Science and Technology [2013CBA01402]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25938]
专题	中科院植物分子生理学重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China 3.Yangzhou Univ, Coll Agr, Jiangsu Key Lab Crop Genet & Physiol, Key Lab Plant Funct Genom,Minist Educ China, Yangzhou 225009, Jiangsu, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Deng, Zhu Yun,Liu, Ling Tong,Li, Tang,et al. OsKinesin-13A Is an Active Microtubule Depolymerase Involved in Glume Length Regulation via Affecting Cell Elongation[J]. SCIENTIFIC REPORTS,2015,5.
APA	Deng, Zhu Yun.,Liu, Ling Tong.,Li, Tang.,Yan, Song.,Kuang, Bai Jian.,...&Wang, Tai.(2015).OsKinesin-13A Is an Active Microtubule Depolymerase Involved in Glume Length Regulation via Affecting Cell Elongation.SCIENTIFIC REPORTS,5.
MLA	Deng, Zhu Yun,et al."OsKinesin-13A Is an Active Microtubule Depolymerase Involved in Glume Length Regulation via Affecting Cell Elongation".SCIENTIFIC REPORTS 5(2015).