Effects of Pyropia yezoensis eYnzymatic hydrolysate on the growth and immune regulation of the zebrafish

doi:10.1016/j.fsi.2022.01.033

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	Effects of Pyropia yezoensis eYnzymatic hydrolysate on the growth and immune regulation of the zebrafish
	Wang, Xiaoqing 1,2,3,4; Liu, Jing 1,2,5; Geng, Lihua 1,2,3; Yang, Yue 1,2,3; Wu, Ning 1,2,3; Zhang, Quanbin 1,2,3; Wang, Jing 1,2,3
刊名	FISH & SHELLFISH IMMUNOLOGY
	2022-03-01
卷号	122 页码:21-28
关键词	Pyropia yezoensis Immune regulation Growth promotion Zebrafish Intestinal flora
ISSN号	1050-4648
DOI	10.1016/j.fsi.2022.01.033
通讯作者	Wang, Jing(jingwang@qdio.ac.cn)
英文摘要	The supplemental effect of Pyropia yezoensis enzymatic hydrolysate (PYE) in fish diet was evaluated in zebrafish (Danio rerio) model. A basal diet supplemented with PYE at 0, 0.1, 1.0 and 2.0% were fed to one-month old zebrafish for 6 weeks, its growth performance and immunity index were evaluated. The increase in weight gain was significantly higher when supplementary 1% PYE which shows a positive effect on growth performance of zebrafish. In addition, crude protein content of fish body was increased in all PYE supplemental groups. The innate immune responses and activity of digestive enzymes in zebrafish were enhanced with dietary supplementation of PYE additives. Compared with the control group, lysozyme (LYZ) and interleukin-10 (IL-10) content in zebrafish intestines were up-regulated in groups fed with 0.1% and 1% PYE. The mRNA expression levels of LYZ and IL-10 in zebrafish intestines were consistent with ELISA results. The content of tumor necrosis factor (TNF-alpha) reduced in 1% and 2% PYE groups. Furthermore, PYE down-regulated the relative abundance of pathogenic bacteria (Aeromonadaceae) and up-regulated the relative abundance of fish probiotics (Brevibacillus) in intestinal flora. The findings in this study indicated that PYE supplementation in diet could promote growth, improve immunity and regulate intestinal flora, which made PYE considered as an potential aquatic additive.
资助项目	National Key Research and Development Program of China[2019YFD0900705]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000819786900003
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/179640]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, Jing
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Wenhai Rd, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Qing Dao Agr Univ, Qingdao 266109, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiaoqing,Liu, Jing,Geng, Lihua,et al. Effects of Pyropia yezoensis eYnzymatic hydrolysate on the growth and immune regulation of the zebrafish[J]. FISH & SHELLFISH IMMUNOLOGY,2022,122:21-28.
APA	Wang, Xiaoqing.,Liu, Jing.,Geng, Lihua.,Yang, Yue.,Wu, Ning.,...&Wang, Jing.(2022).Effects of Pyropia yezoensis eYnzymatic hydrolysate on the growth and immune regulation of the zebrafish.FISH & SHELLFISH IMMUNOLOGY,122,21-28.
MLA	Wang, Xiaoqing,et al."Effects of Pyropia yezoensis eYnzymatic hydrolysate on the growth and immune regulation of the zebrafish".FISH & SHELLFISH IMMUNOLOGY 122(2022):21-28.