Cultivation activities play an important role in shaping the spatial distribution of rock fragments in hilly agricultural landscapes. The spatial variation in rock fragment contents (SVRFC) can exert strong effects on the spatial heterogeneity of soil properties with great implications for hydrological fluxes and soil erosion. However, previous research always assumed that rock fragments were homogenously distributed, but little has been known concerning the SVRFC over the hillslope and its influence on hydrological and erosional processes. In this study, simulated rock fragment contents (RFCs) in soil layers at the upper (0.6 kg kg(- 1)) and lower slope positions (0, 0.2, 0.4 and 0.6 kg kg(-1)) were established in combination with artificial rainfall experiments, to investigate the influence of RFC difference between the upper and lower slope positions on hydrological and erosional processes. Results show that the decreasing RFC difference reduced the yield of surface flow (5-100%), but increased yields of subsurface flow (58-792%) and deep percolation (19-130%). Additionally, the decreasing RFC difference reduced the runoff velocity of surface flow and surface erosion rate, but aggravated subsurface erosion. Especially under the 0 kg kg(-1) RFC difference, the intense soil loss occurred at the subsurface which may be due to the highly developed preferential flow. With increasing rainfall intensity, great increments in yields of subsurface flow, deep percolation and subsurface sediments were found under the small RFC difference which had small increments in yields of surface flow and surface sediments. These results suggest that the changing SVRFC related to tillage erosion can exert strong influence on behaviors of hydrological and erosional processes. Our study provides a better understanding of the interaction between agricultural cultivation and soil-water loss in hilly agricultural landscapes.