Detection of Prokaryotes on the Astomatous Ciliated Protist Kentrophoros flavus (Ciliophora, Karyorelictea) Revealed A Consistently Associated Muribaculaceae-Like Bacterium

doi:10.3389/fmars.2022.879388

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Detection of Prokaryotes on the Astomatous Ciliated Protist Kentrophoros flavus (Ciliophora, Karyorelictea) Revealed A Consistently Associated Muribaculaceae-Like Bacterium
	Bi, Luping 4; Zhang, Xiaoxin 3,4; Zou, Songbao 2; Ji, Daode 4; Zhang, Qianqian 1,3
刊名	FRONTIERS IN MARINE SCIENCE
	2022-06-06
卷号	9 页码:13
关键词	Kentrophoros symbiosis bacterial diversity ciliate Muribaculaceae
DOI	10.3389/fmars.2022.879388
通讯作者	Ji, Daode(daodeji@126.com) ; Zhang, Qianqian(qqzhang@yic.ac.cn)
英文摘要	The interactions between symbiotic bacterial consortia and their protist hosts in benthic environments have attracted increasing interest in recent years. In the present study, we investigated the diversity of potentially associated bacteria for an astomatous ciliate, Kentrophoros flavus, collected in the intertidal zone of Yantai, China. For the first time, the diversity of the associated bacteria in the species K. flavus was examined using 16S rRNA-based techniques (clone libraries and PacBio sequencing) and the fluorescence in situ hybridization (FISH) technique. The 16S rRNA-based sequencing revealed a higher diversity of associated bacteria in K. flavus than previously expected. In addition to a genus-typical thiotrophic symbiont, the "Candidatus Kentron" stain YE, we provide evidence showing the consistent existence of one Muribaculaceae-like bacterium that was secondarily abundant among the bacterial operational taxonomic units (OTUs). Fluorescence in situ hybridization (FISH) with three specific probes and double-label FISH experiments with "Candidatus Kentron" probes showed that the Muribaculaceae-like bacterium was abundant and merged with the "Candidatus Kentron" stain YE on the cell surface of the host. A phylogenetic analysis of the bacterial 16S rRNA gene showed that the bacterium was a distinct branch in Muribaculaceae, members of which are primarily reported from gut microbiome. The name "Muribaculaceae-like bacterium associated with Kentrophoros flavus" (MLAKF) is proposed for the new bacterium. The higher 16S rRNA diversity in K. flavus and the discovery of MLAKF on the cell surface both suggest a potential bacterial consortium that interacts with the host K. flavus.
WOS关键词	SEQUENCE ALIGNMENT ; PHYLOGENY ; YANTAIENSIS ; MORPHOLOGY ; PLANT
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
语种	英语
WOS记录号	WOS:000813310800001
资助机构	National Natural Science Foundation of China ; Youth Innovation Promotion Association, CAS ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Key Research Program of Frontier Science, CAS
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/31026]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者	Ji, Daode; Zhang, Qianqian
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai, Peoples R China 2.Zhejiang Inst Freshwater Fisheries, Key Lab Fish Hlth & Nutr Zhejiang Prov, Huzhou, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai, Peoples R China 4.Yantai Univ, Sch Ocean, Yantai, Peoples R China
推荐引用方式 GB/T 7714	Bi, Luping,Zhang, Xiaoxin,Zou, Songbao,et al. Detection of Prokaryotes on the Astomatous Ciliated Protist Kentrophoros flavus (Ciliophora, Karyorelictea) Revealed A Consistently Associated Muribaculaceae-Like Bacterium[J]. FRONTIERS IN MARINE SCIENCE,2022,9:13.
APA	Bi, Luping,Zhang, Xiaoxin,Zou, Songbao,Ji, Daode,&Zhang, Qianqian.(2022).Detection of Prokaryotes on the Astomatous Ciliated Protist Kentrophoros flavus (Ciliophora, Karyorelictea) Revealed A Consistently Associated Muribaculaceae-Like Bacterium.FRONTIERS IN MARINE SCIENCE,9,13.
MLA	Bi, Luping,et al."Detection of Prokaryotes on the Astomatous Ciliated Protist Kentrophoros flavus (Ciliophora, Karyorelictea) Revealed A Consistently Associated Muribaculaceae-Like Bacterium".FRONTIERS IN MARINE SCIENCE 9(2022):13.