A novel polymer-based nitrocellulose platform for implementing a multiplexed microfluidic paper-based enzyme-linked immunosorbent assay

doi:10.1038/s41378-022-00385-z

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	A novel polymer-based nitrocellulose platform for implementing a multiplexed microfluidic paper-based enzyme-linked immunosorbent assay
	Lin, Dong 4,5,6; Li, Bowei 6; Fu, Longwen 6; Qi, Ji 3,6; Xia, Chunlei 6; Zhang, Yi 2; Chen, Jiadong 1; Choo, Jaebum 1; Chen, Lingxin 3,5,6
刊名	MICROSYSTEMS & NANOENGINEERING
	2022-05-19
卷号	8 期号:1 页码:10
ISSN号	2055-7434
DOI	10.1038/s41378-022-00385-z
通讯作者	Li, Bowei(bwli@yic.ac.cn) ; Choo, Jaebum(jbchoo@cau.ac.kr) ; Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要	Nitrocellulose (NC) membranes, as porous paper-like substrates with high protein-binding capabilities, are very popular in the field of point-of-care immunoassays. However, generating robust hydrophobic structures in NC membranes to fabricate microfluidic paper-based analytical devices (mu PADs) remains a great challenge. At present, the main method relies on an expensive wax printer. In addition, NC membranes very easy to adhere during the printing process due to electrostatic adsorption. Herein, we developed a facile, fast and low-cost strategy to fabricate mu PADs in NC membranes by screen-printing polyurethane acrylate (PUA) as a barrier material for defining flow channels and reaction zones. Moreover, hydrophobic barriers based on UV-curable PUA can resist various surfactant solutions and organic solvents that are generally used in immunoassays and biochemical reactions. To validate the feasibility of this PUA-based NC membrane for immunoassays in point-of-care testing (POCT), we further designed and assembled a rotational paper-based analytical device for implementing a multiplexed enzyme-linked immunosorbent assay (ELISA) in a simple manner. Using the proposed device under the optimal conditions, alpha fetoprotein (AFP) and carcinoembryonic antigen (CEA) could be identified, with limits of detection of 136 pg/mL and 174 pg/mL, respectively, which are below the threshold values of these two cancer biomarkers for clinical diagnosis. We believe that this reliable device provides a promising platform for the diagnosis of disease based on ELISA or other related bioassays in limited settings or remote regions.
WOS关键词	COLORIMETRIC DETECTION ; PATTERNED-PAPER ; DEVICES ; POINT ; FABRICATION ; BARRIERS
WOS研究方向	Science & Technology - Other Topics ; Instruments & Instrumentation
语种	英语
WOS记录号	WOS:000797972800001
资助机构	Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences ; National Natural Science Foundation of China ; Shandong Provincial Natural Science Foundation Key Project ; Shandong Provincial Natural Science Foundation Key Research and Development Project ; National Research Foundation of Korea ; Open Fund of CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/31104]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Li, Bowei; Choo, Jaebum; Chen, Lingxin
作者单位	1.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea 2.Jiangnan Univ, Inst Analyt Food Safety, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 6.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Processes & Ecol Re, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Lin, Dong,Li, Bowei,Fu, Longwen,et al. A novel polymer-based nitrocellulose platform for implementing a multiplexed microfluidic paper-based enzyme-linked immunosorbent assay[J]. MICROSYSTEMS & NANOENGINEERING,2022,8(1):10.
APA	Lin, Dong.,Li, Bowei.,Fu, Longwen.,Qi, Ji.,Xia, Chunlei.,...&Chen, Lingxin.(2022).A novel polymer-based nitrocellulose platform for implementing a multiplexed microfluidic paper-based enzyme-linked immunosorbent assay.MICROSYSTEMS & NANOENGINEERING,8(1),10.
MLA	Lin, Dong,et al."A novel polymer-based nitrocellulose platform for implementing a multiplexed microfluidic paper-based enzyme-linked immunosorbent assay".MICROSYSTEMS & NANOENGINEERING 8.1(2022):10.