Facile fabrication of highly sensitive and non-label aptasensors based on antifouling amyloid-like protein aggregates

doi:10.1039/d2ay00416j

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋腐蚀与防护研究发展中心

	Facile fabrication of highly sensitive and non-label aptasensors based on antifouling amyloid-like protein aggregates
	Ye, Xiangyi 1,3; Zhang, Dun 1,2,3; Zeng, Yan 1,3; Wang, Yingwen 1,2,3; Qi, Peng 1,2,3
刊名	ANALYTICAL METHODS
	2022-05-11
页码	7
ISSN号	1759-9660
DOI	10.1039/d2ay00416j
通讯作者	Zhang, Dun(zhangdun@qdio.ac.cn) ; Qi, Peng(qipeng@qdio.ac.cn)
英文摘要	In this paper, we present a robust and versatile method for developing non-label aptasensors with high sensitivity. Amyloid-like protein aggregates were facilely synthesized with the commonly used passivating agent bovine serum albumin (BSA) in developing biosensors, and the produced amyloid-like phase-transited BSA (PTB) exhibited excellent antifouling performances and robust interfacial adhesion with the electrode surface. In order to improve the detection sensitivity of electrochemical measurements, reduced graphene oxide was electrochemically deposited onto the electrode surface. Moreover, gold nanoparticles were introduced to enhance the conductivity of the PTB film and facilitate subsequent aptamer modification. Two common biological species, adenosine triphosphate (ATP) and cytochrome c (cyt c), were chosen as detection targets, and their corresponding aptasensors were successfully constructed and systematically evaluated. The proposed aptasensors based on the PTB-Au antifouling composite exhibited high sensitivity and specificity towards ATP and cyt c detection, and the detection limits were calculated to be 0.26 nM and 0.64 nM for ATP and cyt c, respectively. Hence, this work provides a simple approach to develop highly sensitive aptasensors without any labeling process, and thus promises its great application in biological analysis.
资助项目	National Natural Science Foundation of China[41876101] ; National Natural Science Foundation of China[41906037] ; Nantong Scientific Plan Foundation[JC2021054] ; Nantong Scientific Plan Foundation[JC2020090]
WOS研究方向	Chemistry ; Food Science & Technology ; Spectroscopy
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000804237200001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/179410]
专题	海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者	Zhang, Dun; Qi, Peng
作者单位	1.Pilot Natl Lab Marine Sci & Technol Qingdao, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Ye, Xiangyi,Zhang, Dun,Zeng, Yan,et al. Facile fabrication of highly sensitive and non-label aptasensors based on antifouling amyloid-like protein aggregates[J]. ANALYTICAL METHODS,2022:7.
APA	Ye, Xiangyi,Zhang, Dun,Zeng, Yan,Wang, Yingwen,&Qi, Peng.(2022).Facile fabrication of highly sensitive and non-label aptasensors based on antifouling amyloid-like protein aggregates.ANALYTICAL METHODS,7.
MLA	Ye, Xiangyi,et al."Facile fabrication of highly sensitive and non-label aptasensors based on antifouling amyloid-like protein aggregates".ANALYTICAL METHODS (2022):7.