Identification and characterization of olfactory receptor genes and olfactory perception in rapa whelk Rapana venosa (Valenciennes, 1846) during larval settlement and metamorphosis

doi:10.1016/j.gene.2022.146403

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Identification and characterization of olfactory receptor genes and olfactory perception in rapa whelk Rapana venosa (Valenciennes, 1846) during larval settlement and metamorphosis
	Yu, Zheng-Lin 1,2; Feng, Jie 2,3,4,7,9; Song, Hao 2,3,4,7,9; Yang, Mei-Jie 2,3,4,7,9; Hu, Zhi 2,3,4,7,8,9; Zhou, Cong 2,3,4,7,8,9; Shi, Pu 2,3,4,7,8,9; Yuan, Xiu-Tang 1,2; Li, Hai-Zhou 6; Zhang, Tao 2,3,4,7,9
刊名	GENE
	2022-05-30
卷号	825 页码:8
关键词	Olfactory perception Rapana venosa OR gene family Settlement and metamorphosis
ISSN号	0378-1119
DOI	10.1016/j.gene.2022.146403
通讯作者	Zhang, Tao(tzhang@qdio.ac.cn)
英文摘要	The rapa whelk Rapana venosa, an economically important marine fishery resource in China but a major invader all over the world, changes from a phytophagous to a carnivorous form following settlement and metamorphosis. However, the low settlement and metamorphosis rates (<1%) of larvae limit the abundance of R. venosa. This critical step (settlement and metamorphosis) remains poorly characterized but may be related to how larvae perceive the presence of shellfish, their new source of food. Here, we report that larvae may use olfactory perception to sense shellfish. Olfactory receptor (OR) genes are involved in odor sensing in animals. We identified a total of 463 OR genes, which could be grouped into nine clades based on phylogenetic analysis. When assessing the attraction of larvae at different developmental stages to oyster odor, R. venosa showed active settlement and metamorphosis behavior only at the J4 stage (competent larva, 1000-1500 mu m shell length) and in the presence of shellfish odor at the same time. Expression of OR gene family members differed between stage 2 (four-spiral whorl stage) and stage 1 (single-to three-spiral whorl stage), indicating significant changes in the olfactory system during larval development. These findings broaden our understanding of olfactory perception, settlement, and metamorphosis in gastropods and can be used to improve R. venosa harvesting, as well as the sustainable development and utilization of this resource.
资助项目	National Natural Science Foundation of China[32002374] ; National Natural Science Foundation of China[32002409] ; National Natural Science Foundation of China[31972814] ; Natural Science Foundation of Shandong Province[ZR2019BD003] ; China Postdoctoral Science Foundation[2019 M652498] ; Major Scientific and Technological Innovation Project of the Shandong Provincial Key Research and Development Program[2019JZZY020708] ; China Agriculture Research System of MOF and MARA ; Industry Leading Talents Project of Taishan Scholars[LJNY201704] ; 'Double Hundred' Blue Industry Leader Team of Yantai ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology[LMEES-CTSP-2018-1]
WOS研究方向	Genetics & Heredity
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000792441100001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/179188]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Zhang, Tao
作者单位	1.Chinese Acad Sci, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 5.Genepioneer Biotechnol Co Ltd, Nanjing 210000, Jiangsu, Peoples R China 6.Shandong Fu Han Ocean Sci Tech Co Ltd, Haiyang 265114, Peoples R China 7.Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 8.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 9.Chinese Acad Sci, CAS Engn Lab Marine Ranching, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Yu, Zheng-Lin,Feng, Jie,Song, Hao,et al. Identification and characterization of olfactory receptor genes and olfactory perception in rapa whelk Rapana venosa (Valenciennes, 1846) during larval settlement and metamorphosis[J]. GENE,2022,825:8.
APA	Yu, Zheng-Lin.,Feng, Jie.,Song, Hao.,Yang, Mei-Jie.,Hu, Zhi.,...&Liu, Lei.(2022).Identification and characterization of olfactory receptor genes and olfactory perception in rapa whelk Rapana venosa (Valenciennes, 1846) during larval settlement and metamorphosis.GENE,825,8.
MLA	Yu, Zheng-Lin,et al."Identification and characterization of olfactory receptor genes and olfactory perception in rapa whelk Rapana venosa (Valenciennes, 1846) during larval settlement and metamorphosis".GENE 825(2022):8.