<p>A marine fungus Alternaria alternata FB1 efficiently degrades polyethylene</p>

doi:10.1016/j.jhazmat.2022.128617

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	A marine fungus Alternaria alternata FB1 efficiently degrades polyethylene
	Gao, Rongrong 2,3,4,5,6; Liu, Rui 1,2,3,4,5; Sun, Chaomin 2,3,4,5,6
刊名	JOURNAL OF HAZARDOUS MATERIALS
	2022-06-05
卷号	431 页码:11
关键词	Ocean Fungus Polyethylene Biodegradation Enzyme
ISSN号	0304-3894
DOI	10.1016/j.jhazmat.2022.128617
通讯作者	Sun, Chaomin(sunchaomin2020@126.com)
英文摘要	Huge quantities of plastic wastes have been accumulating in the environment causing serious ecological prob-lems and significantly impacting the global carbon cycling. Plastic pollutions have been recognized as the most common and durable marine contaminants. Consequently, the marine environment is becoming a hot spot to screen microorganisms possessing potential plastic degradation capabilities. Here, by screening hundreds of plastic waste-associated samples, we isolated a fungus (named Alternaria alternata FB1) that possessing a prominent capability of colonizing on the polyethylene (PE) film. Through Scanning Electron Microscope (SEM) observation, we found this fungus could efficiently degrade the PE film and formed numerous obvious holes in the plastic surface. Moreover, the Fourier Transform Infrared (FTIR) imaging detected absorption peak in the vicinity of 1715 cm(-1), indicating the formation of carbonyl bonds (center dot-C- O--). Through X-Ray Diffraction (XRD) analysis, we found that the PE film treated by strain FB1 for 28 days showed an evident reduced relative crystallinity degree, resulting in a decrease from 62.79% to 52.02%. Strikingly, the molecular weight of PE film decreased 95% after 120 days treatment by strain FB1. Using GC-MS, we further clarified that a four-carbon product (named Diglycolamine) accounted for 93.28% of all degradation products. We defined 153 enzymes that potentially involved in the degradation of PE through a transcriptomic method. The degradation capabilities of two representative enzymes including a laccase (with a molecular weight about 59.49 kDa) and a peroxidase (with a molecular weight about 36.7 kDa) were verified. Lastly, a complete biodegradation process of PE was proposed. Given the extreme paucity of microorganisms and enzymes for effective degradation of PE in the present time, our study provides a compelling candidate for further investigation of degradation mechanisms and development of biodegradation products of PE.& nbsp;
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301] ; China Ocean Mineral Resources R&D Association Grant[DY135-B2-14] ; Major Research Plan of the National Natural Science Foundation[92051107] ; Key Deployment Projects of Center of Ocean Mega-Science of the Chinese Academy of Sciences[COMS2020Q04] ; Shandong Provincial Natural Science Foundation[ZR2021ZD28] ; Taishan Young Scholar Program of Shandong Province[tsqn20161051]
WOS研究方向	Engineering ; Environmental Sciences & Ecology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000782588900002
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/178855]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Sun, Chaomin
作者单位	1.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Peoples R China 5.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 6.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Gao, Rongrong,Liu, Rui,Sun, Chaomin. A marine fungus Alternaria alternata FB1 efficiently degrades polyethylene [J]. JOURNAL OF HAZARDOUS MATERIALS,2022,431:11.
APA	Gao, Rongrong,Liu, Rui,&Sun, Chaomin.(2022). A marine fungus Alternaria alternata FB1 efficiently degrades polyethylene .JOURNAL OF HAZARDOUS MATERIALS,431,11.
MLA	Gao, Rongrong,et al." A marine fungus Alternaria alternata FB1 efficiently degrades polyethylene ".JOURNAL OF HAZARDOUS MATERIALS 431(2022):11.