Characterization of the Dual Functions of LvCrustinVII from Litopenaeus vannamei as Antimicrobial Peptide and Opsonin

doi:10.3390/md20030157

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Characterization of the Dual Functions of LvCrustinVII from Litopenaeus vannamei as Antimicrobial Peptide and Opsonin
	Hu, Jie 2,3,7; Li, Shihao 1,2,3,5; Lv, Qian 2,3,7; Miao, Miao 2,3,4; Li, Xuechun 2,3,4; Li, Fuhua 1,2,3,5,6
刊名	MARINE DRUGS
	2022-03-01
卷号	20 期号:3 页码:13
关键词	antimicrobial peptides (AMPs) crustin antibacterial activity opsonin Litopenaeus vannamei
DOI	10.3390/md20030157
通讯作者	Li, Shihao(lishihao@qdio.ac.cn)
英文摘要	Crustin are a family of antimicrobial peptides that play an important role in protecting against pathogens infection in the innate immune system of crustaceans. Previously, we identified several novel types of crustins, including type VI and type VII crustins. However, their immune functions were still unclear. In the present study, the immune function of type VII crustin LvCrustinVII were investigated in Litopenaeus vannamei. LvCrustinVII was wildly expressed in all tested tissues, with relatively high expression levels in hepatopancreas, epidermis and lymphoid organ. Upon Vibrio parahaemolyticus infection, LvCrustinVII was significantly upregulated in hepatopancreas. Recombinant LvCrustinVII (rLvCrustinVII) showed strong inhibitory activities against Gram-negative bacteria Vibrio harveyi and V. parahaemolyticus, while weak activities against the Gram-positive bacteria Staphylococcus aureus. Binding assay showed that rLvCrustinVII could bind strongly to V. harveyi and V. parahaemolyticus, as well as the cell wall components Glu, LPS and PGN. In the presence of Ca2+, rLvCrustinVII could agglutinate V. parahaemolyticus and enhance hemocyte phagocytosis. The present data partially illustrate the immune function of LvCrustinVII, which enrich our understanding on the functional mechanisms of crustins and provide useful information for application of this kind of antimicrobial peptides.
资助项目	Key Program of the National Natural Science Foundation of China[31830100] ; General Program of National Natural Science Foundation of China[31972829] ; General Program of National Natural Science Foundation of China[41776158] ; China Agriculture Research System of MOF and MARA
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	MDPI
WOS记录号	WOS:000774996200001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/178687]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Li, Shihao
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 2.Chinese Acad Sci, Shandong Prov Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 6.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China 7.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Peoples R China
推荐引用方式 GB/T 7714	Hu, Jie,Li, Shihao,Lv, Qian,et al. Characterization of the Dual Functions of LvCrustinVII from Litopenaeus vannamei as Antimicrobial Peptide and Opsonin[J]. MARINE DRUGS,2022,20(3):13.
APA	Hu, Jie,Li, Shihao,Lv, Qian,Miao, Miao,Li, Xuechun,&Li, Fuhua.(2022).Characterization of the Dual Functions of LvCrustinVII from Litopenaeus vannamei as Antimicrobial Peptide and Opsonin.MARINE DRUGS,20(3),13.
MLA	Hu, Jie,et al."Characterization of the Dual Functions of LvCrustinVII from Litopenaeus vannamei as Antimicrobial Peptide and Opsonin".MARINE DRUGS 20.3(2022):13.