Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds

doi:10.1016/j.jplph.2016.02.021

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds
	Liu, Shu-Jun ; Xu, Heng-Heng ; Wang, Wei-Qing ; Li, Ni ; Wang, Wei-Ping ; Lu, Zhuang ; Moller, Ian Max 1; Song, Song-Quan
刊名	JOURNAL OF PLANT PHYSIOLOGY
	2016
卷号	196-197 页码:79-92
关键词	Embryo Oryza sativa Proteome analysis Seed germination Seedling establishment
ISSN号	0176-1617
DOI	10.1016/j.jplph.2016.02.021
文献子类	Article
英文摘要	Seed germination is a critical phase in the plant life cycle, but the mechanism of seed germination is still poorly understood. In the present study, rice (Oryza sativa L. cv. Peiai 64S) seeds were sampled individually when they reached different germination stages, quiescent, germinated sensu stricto, germinated completely and seedling, and were used to study the changes in the embryo proteome. A total of 88 protein spots showed a significant change in abundance during germination in water, and the results showed an activation of metabolic processes. Cell division, cell wall synthesis, and secondary metabolism were activated at late seed germination and during preparation for subsequent seedling establishment. Cycloheximide (CHX) at 70 mu M inhibited seedling establishment without an apparent negative effect on seed germination, while CHX at 500 mu M completely blocked seed germination. We used this observation to identify the potentially important proteins involved in seed germination (coleoptile protrusion) and seedling establishment (coleoptile and radicle protrusion). Twenty-six protein spots, mainly associated with sugar/polysaccharide metabolism and energy production, showed a significant difference in abundance during seed germination. Forty-nine protein spots, mainly involved in cell wall biosynthesis, proteolysis as well as cell defense and rescue, were required for seedling establishment. The results help improve our understanding of the key events (proteins) involved in germination and seedling development. (C) 2016 Elsevier GmbH. All rights reserved.
学科主题	Plant Sciences
电子版国际标准刊号	1618-1328
出版地	MUNICH
WOS关键词	PROTEOMIC ANALYSIS ; MOLECULAR-CLONING ; GENE-EXPRESSION ; MESSENGER-RNAS ; LIPOXYGENASE ; MOBILIZATION ; GROWTH ; ROLES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	ELSEVIER GMBH
WOS记录号	WOS:000376162400009
资助机构	National Science and Technology Support Program [2012BAC01B05] ; Chinese Academy of Sciences Visiting Professorship for senior international scientists
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25246]
专题	中科院北方资源植物重点实验室
作者单位	1.Hunan Hybrid Rice Res Center, State Key Lab Hybrid Rice, Changsha 410125, Hunan, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China 4.Aarhus Univ, Dept Mol Biol & Genet, DK-4200 Slagelse, Denmark
推荐引用方式 GB/T 7714	Liu, Shu-Jun,Xu, Heng-Heng,Wang, Wei-Qing,et al. Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds[J]. JOURNAL OF PLANT PHYSIOLOGY,2016,196-197:79-92.
APA	Liu, Shu-Jun.,Xu, Heng-Heng.,Wang, Wei-Qing.,Li, Ni.,Wang, Wei-Ping.,...&Song, Song-Quan.(2016).Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds.JOURNAL OF PLANT PHYSIOLOGY,196-197,79-92.
MLA	Liu, Shu-Jun,et al."Identification of embryo proteins associated with seed germination and seedling establishment in germinating rice seeds".JOURNAL OF PLANT PHYSIOLOGY 196-197(2016):79-92.