Global transcriptome profiling analysis reveals insight into saliva-responsive genes in alfalfa

doi:10.1007/s00299-015-1903-9

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	Global transcriptome profiling analysis reveals insight into saliva-responsive genes in alfalfa
	Liu, Wenxian ; Zhang, Zhengshe ; Chen, Shuangyan 2; Ma, Lichao ; Wang, Hucheng ; Dong, Rui ; Wang, Yanrong ; Liu, Zhipeng
刊名	PLANT CELL REPORTS
	2016
卷号	35 期号:3 页码:561-571
关键词	Alfalfa Next-generation sequencing Saliva deposition Differentially expressed genes Transcriptomes
ISSN号	0721-7714
DOI	10.1007/s00299-015-1903-9
文献子类	Article
英文摘要	Saliva deposition is one of the key factors influencing plant-herbivore interactions during grazing. Although many studies have focused on the effects of saliva deposition on plant regrowth, no consistent conclusions have been reached. Alfalfa is the most extensively cultivated forage legume, yet most alfalfa cultivars, thus far, are not grazing-tolerant. To better understand the underlying mechanism, we undertook a study to evaluate the global changes in the transcriptome of alfalfa after cow saliva deposition treatment. In this study, cDNA libraries from alfalfa seedlings at 0, 4, 8, and 24 h after cow saliva deposition were constructed and sequenced, resulting in the identification of 53,195 annotated unigenes, from which 4,814 unigenes were significantly differentially expressed. A metabolic pathway enrichment analysis demonstrated that saliva deposition functions as a stress that negatively affects the regrowth of alfalfa by modifying jasmonic acid synthesis, enhancing the susceptibility to pathogens and reducing the expression levels of ribosomal protein genes. In the present study, we demonstrate the potential effects of saliva deposition on alfalfa regrowth at the transcriptome level. These fundamental and important findings could facilitate further investigations into the molecular mechanisms underlying the responses of alfalfa and other related species to herbivore grazing.
学科主题	Plant Sciences
电子版国际标准刊号	1432-203X
出版地	NEW YORK
WOS关键词	DIFFERENTIALLY EXPRESSED GENES ; RNA-SEQ ; GROWTH-RESPONSES ; ANIMAL SALIVA ; PLANT-GROWTH ; SMALL AUXIN ; TOOL ; PROTEIN ; IDENTIFICATION ; COMPENSATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000371241100006
资助机构	National Basic Research Program of ChinaNational Basic Research Program of China [2014CB138704] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31272492, 31502000] ; Program for Changjiang Scholars and Innovative Research Team in UniversityProgram for Changjiang Scholars & Innovative Research Team in University (PCSIRT) [IRT13019]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25241]
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Lanzhou Univ, Coll Pastoral Agr Sci & Technol, State Key Lab Grassland Agroecosyst, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Liu, Wenxian,Zhang, Zhengshe,Chen, Shuangyan,et al. Global transcriptome profiling analysis reveals insight into saliva-responsive genes in alfalfa[J]. PLANT CELL REPORTS,2016,35(3):561-571.
APA	Liu, Wenxian.,Zhang, Zhengshe.,Chen, Shuangyan.,Ma, Lichao.,Wang, Hucheng.,...&Liu, Zhipeng.(2016).Global transcriptome profiling analysis reveals insight into saliva-responsive genes in alfalfa.PLANT CELL REPORTS,35(3),561-571.
MLA	Liu, Wenxian,et al."Global transcriptome profiling analysis reveals insight into saliva-responsive genes in alfalfa".PLANT CELL REPORTS 35.3(2016):561-571.