New Insights on Drought Stress Response by Global Investigation of Gene Expression Changes in Sheepgrass (Leymus chinensis)

doi:10.3389/fpls.2016.00954

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	New Insights on Drought Stress Response by Global Investigation of Gene Expression Changes in Sheepgrass (Leymus chinensis)
	Zhao, Pincang 2; Liu, Panpan 2; Yuan, Guangxiao 2; Jia, Junting 2; Li, Xiaoxia ; Qi, Dongmei ; Chen, Shuangyan ; Ma, Tian ; Liu, Gongshe ; Cheng, Liqin
刊名	FRONTIERS IN PLANT SCIENCE
	2016
卷号	7
关键词	sheepgrass drought stress RNA-seq ABA-dependent pathway transcription factors
ISSN号	1664-462X
DOI	10.3389/fpls.2016.00954
文献子类	Article
英文摘要	Water is a critical environmental factor that restricts the geographic distribution of plants. Sheepgrass [Leyrnus chinensis, (Trin.) Tzvel] is an important forage grass in the Eurasia Steppe and a close germplasm for wheat and barley. This native grass adapts well to adverse environments such as cold, salinity, alkalinity and drought, and it can survive when the soil moisture may be less than 6% in dry seasons. However, little is known about how sheepgrass tolerates water stress at the molecular level. Here, drought stress experiment and RNA-sequencing (RNA-seq) was performed in three pools of RNA samples (control, drought stress, and rewatering). We found that sheepgrass seedlings could still survive when the soil water content (SWC) was reduced to 14.09%. Differentially expressed genes (DEGs) analysis showed that 7320 genes exhibited significant responses to drought stress. Of these DEGs, 2671 presented opposite expression trends before and after rewatering. Furthermore, 680 putative sheepgrass-specific water responsive genes were revealed that can be studied deeply. Gene ontology (GO) annotation revealed that stress-associated genes were activated extensively by drought treatment. Interestingly, cold stress-related genes were up-regulated greatly after drought stress. The DEGs of MAPK and calcium signal pathways, plant hormone ABA, jasmonate, ethylene, brassinosteroid signal pathways, cold response CBF pathway participated coordinatively in sheepgrass drought stress response. In addition, we identified 288 putative transcription factors (TFs) involved in drought response, among them, the WRKY, NAG, AP2/ERF, bHLH, bZIP, and MYB families were enriched, and might play crucial and significant roles in drought stress response of sheepgrass. Our research provided new and valuable information for understanding the mechanism of drought tolerance in sheepgrass. Moreover, the identification of genes involved in drought response can facilitate the genetic improvement of crops by molecular breeding.
学科主题	Plant Sciences
出版地	LAUSANNE
WOS关键词	TRANSCRIPTOME-WIDE IDENTIFICATION ; OXYGEN SPECIES PRODUCTION ; SALT-INDUCED GENE ; ABSCISIC-ACID ; RNA-SEQ ; ARABIDOPSIS-THALIANA ; METHYL JASMONATE ; TREHALOSE BIOSYNTHESIS ; TRANSGENIC ARABIDOPSIS ; SIGNAL-TRANSDUCTION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000378849500001
资助机构	National Basic Research Program of China (973 program)National Basic Research Program of China [2014CB138704] ; Project of Science and Technology Service Network Initiative of the Chinese Academy of Sciences (STS) [KFJ-EW-STS-119] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31170316] ; Ministry of Agriculture of ChinaMinistry of Agriculture, China [2014ZX08009-003-002]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25239]
专题	中科院北方资源植物重点实验室
作者单位	1.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Pincang,Liu, Panpan,Yuan, Guangxiao,et al. New Insights on Drought Stress Response by Global Investigation of Gene Expression Changes in Sheepgrass (Leymus chinensis)[J]. FRONTIERS IN PLANT SCIENCE,2016,7.
APA	Zhao, Pincang.,Liu, Panpan.,Yuan, Guangxiao.,Jia, Junting.,Li, Xiaoxia.,...&Cheng, Liqin.(2016).New Insights on Drought Stress Response by Global Investigation of Gene Expression Changes in Sheepgrass (Leymus chinensis).FRONTIERS IN PLANT SCIENCE,7.
MLA	Zhao, Pincang,et al."New Insights on Drought Stress Response by Global Investigation of Gene Expression Changes in Sheepgrass (Leymus chinensis)".FRONTIERS IN PLANT SCIENCE 7(2016).