Proteomic Analysis Reveals the Leaf Color Regulation Mechanism in Chimera Hosta Gold Standard Leaves

doi:10.3390/ijms17030346

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	Proteomic Analysis Reveals the Leaf Color Regulation Mechanism in Chimera Hosta Gold Standard Leaves
	Yu, Juanjuan 2; Zhang, Jinzheng 3; Zhao, Qi ; Liu, Yuelu 3; Chen, Sixue 4; Guo, Hongliang 1; Shi, Lei 3; Dai, Shaojun
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2016
卷号	17 期号:3
关键词	variegated leaves Hosta Gold Standard excess nitrogen fertilization proteomics leaf color
ISSN号	1422-0067
DOI	10.3390/ijms17030346
文献子类	Article
英文摘要	Leaf color change of variegated leaves from chimera species is regulated by fine-tuned molecular mechanisms. Hosta Gold Standard is a typical chimera Hosta species with golden-green variegated leaves, which is an ideal material to investigate the molecular mechanisms of leaf variegation. In this study, the margin and center regions of young and mature leaves from Hosta Gold Standard, as well as the leaves from plants after excess nitrogen fertilization were studied using physiological and comparative proteomic approaches. We identified 31 differentially expressed proteins in various regions and development stages of variegated leaves. Some of them may be related to the leaf color regulation in Hosta Gold Standard. For example, cytosolic glutamine synthetase (GS1), heat shock protein 70 (Hsp70), and chloroplastic elongation factor G (cpEF-G) were involved in pigment-related nitrogen synthesis as well as protein synthesis and processing. By integrating the proteomics data with physiological results, we revealed the metabolic patterns of nitrogen metabolism, photosynthesis, energy supply, as well as chloroplast protein synthesis, import and processing in various leaf regions at different development stages. Additionally, chloroplast-localized proteoforms involved in nitrogen metabolism, photosynthesis and protein processing implied that post-translational modifications were crucial for leaf color regulation. These results provide new clues toward understanding the mechanisms of leaf color regulation in variegated leaves.
学科主题	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
出版地	BASEL
WOS关键词	CYTOSOLIC GLUTAMINE-SYNTHETASE ; DEFICIENT AUREA MUTANT ; VARIEGATED LEAVES ; BLUE-LIGHT ; CHLOROPLAST-BIOGENESIS ; NITROGEN ASSIMILATION ; MOLECULAR ANALYSIS ; PROTEIN IMPORT ; NUCLEAR GENES ; HIGHER-PLANTS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	MDPI
WOS记录号	WOS:000373712800047
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270310] ; Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning ; Capacity Construction Project of Local Universities, Shanghai, China [14390502700] ; Knowledge Innovation Program of the Chinese Academy of ScienceKnowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-B-05, KSCX2-EW-B-02] ; Science and Technology Basic Work of Science and Technology [2014F210300] ; Funding Program for Young Teachers at Universities and Colleges of Shanghai ; General Scientific Research Project of Shanghai Normal University [SK201419]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/24980]
专题	中科院北方资源植物重点实验室
作者单位	1.Univ Florida, Genet Inst, Dept Biol, Interdisciplinary Ctr Biotechnol Res,Plant Mol &, Gainesville, FL 32610 USA 2.Shanghai Normal Univ, Coll Life & Environm Sci, Dev Ctr Plant Germplasm Resources, Shanghai 200234, Peoples R China 3.Northeast Forestry Univ, Alkali Soil Nat Environm Sci Ctr, Key Lab Saline Alkali Vegetat Ecol Restorat Oil F, Minist Educ, Harbin 150040, Peoples R China 4.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 5.Harbin Univ Commerce, Food Engn Coll, Harbin 150028, Peoples R China
推荐引用方式 GB/T 7714	Yu, Juanjuan,Zhang, Jinzheng,Zhao, Qi,et al. Proteomic Analysis Reveals the Leaf Color Regulation Mechanism in Chimera Hosta Gold Standard Leaves[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016,17(3).
APA	Yu, Juanjuan.,Zhang, Jinzheng.,Zhao, Qi.,Liu, Yuelu.,Chen, Sixue.,...&Dai, Shaojun.(2016).Proteomic Analysis Reveals the Leaf Color Regulation Mechanism in Chimera Hosta Gold Standard Leaves.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,17(3).
MLA	Yu, Juanjuan,et al."Proteomic Analysis Reveals the Leaf Color Regulation Mechanism in Chimera Hosta Gold Standard Leaves".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 17.3(2016).