The anaphase-promoting complex initiates zygote division in Arabidopsis through degradation of cyclin B1

doi:10.1111/tpj.13158

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	The anaphase-promoting complex initiates zygote division in Arabidopsis through degradation of cyclin B1
	Guo, Lei 1; Jiang, Li 4; Zhang, Ying ; Lu, Xiu-Li ; Xie, Qi 2; Weijers, Dolf 3; Liu, Chun-Ming
刊名	PLANT JOURNAL
	2016
卷号	86 期号:2 页码:161-174
关键词	APC11 cyclosome zygote activation cell cycle ubiquitination Arabidopsis thaliana
ISSN号	0960-7412
DOI	10.1111/tpj.13158
文献子类	Article
英文摘要	As the start of a new life cycle, activation of the first division of the zygote is a critical event in both plants and animals. Because the zygote in plants is difficult to access, our understanding of how this process is achieved remains poor. Here we report genetic and cell biological analyses of the zygote-arrest 1 (zyg1) mutant in Arabidopsis, which showed zygote-lethal and over-accumulation of cyclin B1 D-box-GUS in ovules. Map-based cloning showed that ZYG1 encodes the anaphase-promoting complex/cyclosome (APC/C) subunit 11 (APC11). Live-cell imaging studies showed that APC11 is expressed in both egg and sperm cells, in zygotes and during early embryogenesis. Using a GFP-APC11 fusion construct that fully complements zyg1, we showed that GFP-APC11 expression persisted throughout the mitotic cell cycle, and localized to cell plates during cytokinesis. Expression of non-degradable cyclin B1 in the zygote, or mutations of either APC1 or APC4, also led to a zyg1-like phenotype. Biochemical studies showed that APC11 has self-ubiquitination activity and is able to ubiquitinate cyclin B1 and promote degradation of cyclin B1. These results together suggest that APC/C-mediated degradation of cyclin B1 in Arabidopsis is critical for initiating the first division of the zygote.
学科主题	Plant Sciences
电子版国际标准刊号	1365-313X
出版地	HOBOKEN
WOS关键词	FEMALE GAMETOGENESIS ; SEED DEVELOPMENT ; CELL-CYCLE ; PROTEIN ; ENCODES ; EMBRYO ; APC/C ; COMPLEX/CYCLOSOME ; EMBRYOGENESIS ; TRANSITION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	WILEY
WOS记录号	WOS:000375103400004
资助机构	National Basic Research Program of ChinaNational Basic Research Program of China [2014CB943401, 2013CB967301] ; Sino-Dutch International Collaboration Project [31161130531]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25339]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 4.Wageningen Univ, Biochem Lab, Dreijenlaan 3, NL-6703 HA Wageningen, Netherlands 5.Chinese Acad Fishery Sci, Beijing 100141, Peoples R China
推荐引用方式 GB/T 7714	Guo, Lei,Jiang, Li,Zhang, Ying,et al. The anaphase-promoting complex initiates zygote division in Arabidopsis through degradation of cyclin B1[J]. PLANT JOURNAL,2016,86(2):161-174.
APA	Guo, Lei.,Jiang, Li.,Zhang, Ying.,Lu, Xiu-Li.,Xie, Qi.,...&Liu, Chun-Ming.(2016).The anaphase-promoting complex initiates zygote division in Arabidopsis through degradation of cyclin B1.PLANT JOURNAL,86(2),161-174.
MLA	Guo, Lei,et al."The anaphase-promoting complex initiates zygote division in Arabidopsis through degradation of cyclin B1".PLANT JOURNAL 86.2(2016):161-174.