MAPK-mediated enhanced expression of vacuolar H+-ATPase confers the improved adaption to NaCl stress in a halotolerate peppermint (Mentha piperita L.)

doi:10.1007/s00709-015-0834-1

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	MAPK-mediated enhanced expression of vacuolar H+-ATPase confers the improved adaption to NaCl stress in a halotolerate peppermint (Mentha piperita L.)
	Li, Zhe ; Zhen, Zhen 2; Guo, Kai ; Harvey, Paul 1; Li, Jishun ; Yang, Hetong
刊名	PROTOPLASMA
	2016
卷号	253 期号:2 页码:553-569
关键词	Vacuolar H+-ATPase H+ pumping activity Na+ compartmentalization MAPK cascade Calcium Peppermint
ISSN号	0033-183X
DOI	10.1007/s00709-015-0834-1
文献子类	Article
英文摘要	Vacuolar H+-ATPase (V-H+-ATPase) has been proved to be of importance in maintenance of ion homeostasis inside plant cells under NaCl stress. In this study, the expression levels and salt-tolerate function of V-H+-ATPase genes were investigated in the roots and leaves of a halotolerate peppermint (Mentha x piperita L.) Keyuan-1 treated with different concentrations of NaCl. Results showed that the expressions of V-H+-ATPase in the transcriptional, protein and activity levels were significantly enhanced in the halotolerate peppermint Keyuan-1 compared to the wild-type (WT) peppermint under 50, 100, and 150 mM NaCl treatment. Moreover, inhibition experiments exhibited that V-H+-ATPase activity played vital roles in the salt tolerance of peppermint Keyuan-1 to 150 mM NaCl stress through increasing the vacuolar H+ pumping activity and Na+ compartmentalization capacity. Furthermore, results of Western blots showed that the activity of a mitogen-activated protein kinase (MAPK) was significantly increased under different concentrations of NaCl in the halotolerate peppermint Keyuan-1, which was much higher than that of WT peppermint. Further experiments with inhibitors suggested that this MAPK protein was responsible for the enhanced expression of V-H+-ATPase in the halotolerate peppermint Keyuan-1. In response to NaCl stress, increase of cytoplasmic calcium concentration ([Ca2+](cyt)) occurred upstream of MAPK activation in the halotolerate peppermint Keyuan-1. In all, these findings demonstrated that increased V-H+-ATPase activity was positively correlated with the enhanced salt tolerance in the halotolerate peppermint Keyuan-1, providing the theoretic basis for the further development and utilization of peppermint in saline areas.
学科主题	Plant Sciences ; Cell Biology
电子版国际标准刊号	1615-6102
出版地	WIEN
WOS关键词	PYROPHOSPHATASE ACTIVITIES ; ALUMINUM PHYTOTOXICITY ; NA+/H+ ANTIPORTER ; PROTEIN-KINASE ; SALT TOLERANCE ; CALCIUM ; FLUORESCENCE ; TRANSPORT ; DROUGHT ; GROWTH
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	SPRINGER WIEN
WOS记录号	WOS:000373578100028
资助机构	Programs for International S&T Cooperation Program of China [2011DFA30990, 2007DFA30630] ; Shandong Provincial Natural Science FoundationNatural Science Foundation of Shandong Province [2014ZRC01073, ZR2014YL002] ; Youth Science Funds of Shandong Academy of Sciences [2013QN012, 2014QN017, 2014QN019] ; Taishan Scholars Program for Overseas Experts of Shandong Province ; National High Technology Research and Development Program (863 Program) of ChinaNational High Technology Research and Development Program of China [2011AA10A205] ; Special Foundation for the Basic Work of Science and Technology [2014FY120900] ; Special Fund for Agro-scientific Research in the Public Interest [201303025]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/24948]
专题	植被与环境变化国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 2.Shandong Acad Sci, Ctr Biotechnol, Jinan 250014, Peoples R China 3.Commonwealth Sci & Ind Res Org, Div Ecosyst Sci, Clayton, Vic 3169, Australia
推荐引用方式 GB/T 7714	Li, Zhe,Zhen, Zhen,Guo, Kai,et al. MAPK-mediated enhanced expression of vacuolar H+-ATPase confers the improved adaption to NaCl stress in a halotolerate peppermint (Mentha piperita L.)[J]. PROTOPLASMA,2016,253(2):553-569.
APA	Li, Zhe,Zhen, Zhen,Guo, Kai,Harvey, Paul,Li, Jishun,&Yang, Hetong.(2016).MAPK-mediated enhanced expression of vacuolar H+-ATPase confers the improved adaption to NaCl stress in a halotolerate peppermint (Mentha piperita L.).PROTOPLASMA,253(2),553-569.
MLA	Li, Zhe,et al."MAPK-mediated enhanced expression of vacuolar H+-ATPase confers the improved adaption to NaCl stress in a halotolerate peppermint (Mentha piperita L.)".PROTOPLASMA 253.2(2016):553-569.