Aquaporin8 regulates cellular development and reactive oxygen species production, a critical component of virulence in Botrytis cinerea

doi:10.1111/nph.13721

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	Aquaporin8 regulates cellular development and reactive oxygen species production, a critical component of virulence in Botrytis cinerea
	An, Bang 1; Li, Boqiang ; Li, Hua 1; Zhang, Zhanquan ; Qin, Guozheng ; Tian, Shiping 1
刊名	NEW PHYTOLOGIST
	2016
卷号	209 期号:4
关键词	aquaporin Botrytis cinerea cellular development reactive oxygen species (ROS) pathogenesis
ISSN号	0028-646X
DOI	10.1111/nph.13721
文献子类	Article
英文摘要	Aquaporins (AQPs) are ubiquitous in nearly all organisms, mediating selective and rapid flux of water across biological membranes. The role of AQPs in phytopathogenic fungi is poorly understood. Orthologs of AQP genes in Botrytis cinerea were identified and knocked out. The effects of AQPs on hyphal growth and conidiation, formation of infection structures and virulence on plant hosts were examined. The role of AQP8 in reactive oxygen species (ROS) production, distribution and transport were further determined. Among eight AQPs, only AQP8 was essential for the ability of B. cinerea to infect plants. AQP8 was demonstrated to be an intrinsic plasma membrane protein, which may function as a channel and mediate hydrogen peroxide uptake. Deletion of AQP8 in B. cinerea completely inhibited the development of conidia and infection structures, and significantly affected noxR expression. Further observations revealed that both AQP8 and noxR impacted ROS distribution in the hyphal tips of B. cinerea. Moreover, AQP8 affected the expression of a mitochondrial protein, NQO1. A knockout mutant of NQO1 was observed to display reduced virulence. These data lead to a better understanding of the important role of AQP8 in the development and pathogenesis of plant pathogens.
学科主题	Plant Sciences
电子版国际标准刊号	1469-8137
出版地	HOBOKEN
WOS关键词	FUNCTIONAL-ANALYSIS ; NADPH OXIDASES ; HYDROGEN-PEROXIDE ; OXIDATIVE STRESS ; GENE FAMILY ; EXPRESSION ; PROTEINS ; PATHOGENICITY ; MYCORRHIZAL ; PLANTS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	WILEY
WOS记录号	WOS:000373379800033
资助机构	National Basic Research Program of ChinaNational Basic Research Program of China [2013CB127103] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31530057, 31172004]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/24621]
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	An, Bang,Li, Boqiang,Li, Hua,et al. Aquaporin8 regulates cellular development and reactive oxygen species production, a critical component of virulence in Botrytis cinerea[J]. NEW PHYTOLOGIST,2016,209(4).
APA	An, Bang,Li, Boqiang,Li, Hua,Zhang, Zhanquan,Qin, Guozheng,&Tian, Shiping.(2016).Aquaporin8 regulates cellular development and reactive oxygen species production, a critical component of virulence in Botrytis cinerea.NEW PHYTOLOGIST,209(4).
MLA	An, Bang,et al."Aquaporin8 regulates cellular development and reactive oxygen species production, a critical component of virulence in Botrytis cinerea".NEW PHYTOLOGIST 209.4(2016).