Quantification of Membrane Protein Dynamics and Interactions in Plant Cells by Fluorescence Correlation Spectroscopy

doi:10.1016/j.molp.2016.06.017

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	Quantification of Membrane Protein Dynamics and Interactions in Plant Cells by Fluorescence Correlation Spectroscopy
	Li, Xiaojuan ; Xing, Jingjing 1,5; Qiu, Zongbo 2; He, Qihua 3; Lin, Jinxing
刊名	MOLECULAR PLANT
	2016
卷号	9 期号:9
关键词	fluorescence correlation spectroscopy microdomain membrane protein dynamics interaction
ISSN号	1674-2052
DOI	10.1016/j.molp.2016.06.017
文献子类	Review
英文摘要	Deciphering the dynamics of protein and lipid molecules on appropriate spatial and temporal scales may shed light on protein function and membrane organization. However, traditional bulk approaches cannot unambiguously quantify the extremely diverse mobility and interactions of proteins in living cells. Fluorescence correlation spectroscopy (FCS) is a powerful technique to describe events that occur at the single-molecule level and on the nanosecond to second timescales; therefore, FCS can provide data on the heterogeneous organization of membrane systems. FCS can also be combined with other microscopy techniques, such as super-resolution techniques. More importantly, FCS is minimally invasive, which makes it an ideal approach to detect the heterogeneous distribution and dynamics of key proteins during development. In this review, we give a brief introduction about the development of FCS and summarize the significant contributions of FCS in understanding the organization of plant cell membranes and the dynamics and interactions of membrane proteins. We also discuss the potential applications of this technique in plant biology.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences
电子版国际标准刊号	1752-9867
出版地	CAMBRIDGE
WOS关键词	CROSS-CORRELATION SPECTROSCOPY ; PLASMA-MEMBRANE ; LIPID RAFTS ; ANOMALOUS DIFFUSION ; LIVING CELLS ; REVEALS ; MICRODOMAINS ; RECEPTOR ; CLATHRIN ; FCS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	CELL PRESS
WOS记录号	WOS:000386585500001
资助机构	State Key Program of National Natural Science of ChinaNational Natural Science Foundation of China (NSFC) [31530084] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370212, 31270224] ; Program of Introducing Talents of Discipline to UniversitiesMinistry of Education, China - 111 Project [13007] ; Program for Changjiang Scholars and Innovative Research Team in UniversityProgram for Changjiang Scholars & Innovative Research Team in University (PCSIRT) [IRT13047]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/24609]
专题	中科院北方资源植物重点实验室
作者单位	1.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 3.Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Peoples R China 4.Peking Univ, Hlth Sci Ctr, Beijing 100191, Peoples R China 5.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Li, Xiaojuan,Xing, Jingjing,Qiu, Zongbo,et al. Quantification of Membrane Protein Dynamics and Interactions in Plant Cells by Fluorescence Correlation Spectroscopy[J]. MOLECULAR PLANT,2016,9(9).
APA	Li, Xiaojuan,Xing, Jingjing,Qiu, Zongbo,He, Qihua,&Lin, Jinxing.(2016).Quantification of Membrane Protein Dynamics and Interactions in Plant Cells by Fluorescence Correlation Spectroscopy.MOLECULAR PLANT,9(9).
MLA	Li, Xiaojuan,et al."Quantification of Membrane Protein Dynamics and Interactions in Plant Cells by Fluorescence Correlation Spectroscopy".MOLECULAR PLANT 9.9(2016).