Proteomic Analysis Reveals Different Involvement of Embryo and Endosperm Proteins during Aging of Yliangyou 2 Hybrid Rice Seeds

doi:10.3389/fpls.2016.01394

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	Proteomic Analysis Reveals Different Involvement of Embryo and Endosperm Proteins during Aging of Yliangyou 2 Hybrid Rice Seeds
	Zhang, Ying-Xue ; Xu, Heng-Heng ; Liu, Shu-Jun ; Li, Ni 1; Wang, Wei-Qing ; Moller, Ian M.; Song, Song-Quan
刊名	FRONTIERS IN PLANT SCIENCE
	2016
卷号	7
关键词	Yliangyou 2 hybrid rice proteome embryo endosperm seed aging
ISSN号	1664-462X
DOI	10.3389/fpls.2016.01394
文献子类	Article
英文摘要	Seed aging is a process that results in a delayed germination, a decreased germination percentage, and finally a total loss of seed viability. However, the mechanism of seed aging is poorly understood. In the present study, Yliangyou 2 hybrid rice (Oryza sativa L.) seeds were artificially aged at 100% relative humidity and 40 degrees C, and the effect of artificial aging on germination, germination time course and the change in protein profiles of embryo and endosperm was studied to understand the molecular mechanism behind seed aging. With an increasing duration of artificial aging, the germination percentage and germination rate of hybrid rice seeds decreased. By comparing the protein profiles from the seeds aged for 0, 10 and 25 days, a total of 91 and 100 protein spots were found to show a significant change of more than 2-fold (P < 0.05) in abundance, and 71 and 79 protein spots were identified, in embryos and endosperms, respectively. The great majority of these proteins increased in abundance in embryos (95%) and decreased in abundance in endosperms (99%). In embryos, most of the identified proteins were associated with energy (30%), with cell defense and rescue (28%), and with storage protein (18%). In endosperms, most of the identified proteins were involved in metabolism (37%), in energy (27%), and in protein synthesis and destination (11%). The most marked change was the increased abundance of many glycolytic enzymes together with the two fermentation enzymes pyruvate decarboxylase and alcohol dehydrogenase in the embryos during aging. We hypothesize that the decreased viability of hybrid rice seeds during artificial aging is caused by the development of hypoxic conditions in the embryos followed by ethanol accumulation.
学科主题	Plant Sciences
出版地	LAUSANNE
WOS关键词	WIDE CHARACTERIZATION ; ARABIDOPSIS-THALIANA ; SUGAR-BEET ; SATIVA L. ; VIGOR ; DETERIORATION ; GERMINATION ; BIOSYNTHESIS ; EXPRESSION ; MUTANTS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000383532100001
资助机构	National Science and Technology Support Program [2012BAC01B05] ; Chinese Academy of SciencesChinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/24597]
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Moller, Ian M.] Aarhus Univ, Dept Mol Biol & Genet, Flakkebjerg, Denmark 3.Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Ying-Xue,Xu, Heng-Heng,Liu, Shu-Jun,et al. Proteomic Analysis Reveals Different Involvement of Embryo and Endosperm Proteins during Aging of Yliangyou 2 Hybrid Rice Seeds[J]. FRONTIERS IN PLANT SCIENCE,2016,7.
APA	Zhang, Ying-Xue.,Xu, Heng-Heng.,Liu, Shu-Jun.,Li, Ni.,Wang, Wei-Qing.,...&Song, Song-Quan.(2016).Proteomic Analysis Reveals Different Involvement of Embryo and Endosperm Proteins during Aging of Yliangyou 2 Hybrid Rice Seeds.FRONTIERS IN PLANT SCIENCE,7.
MLA	Zhang, Ying-Xue,et al."Proteomic Analysis Reveals Different Involvement of Embryo and Endosperm Proteins during Aging of Yliangyou 2 Hybrid Rice Seeds".FRONTIERS IN PLANT SCIENCE 7(2016).