Crystal structure of methylesterase family member 16 (MES16) from Arabidopsis thaliana

doi:10.1016/j.bbrc.2016.04.115

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	Crystal structure of methylesterase family member 16 (MES16) from Arabidopsis thaliana
	Li, Hongmei ; Pu, Hua 1
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2016
卷号	474 期号:1
关键词	Arabidopsis thaliana Chlorophyll breakdown MES16 Demethylation alpha/beta-hydrolase superfamily Catalytic triad
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2016.04.115
文献子类	Article
英文摘要	Methylesterase family member 16 (MES16) is an integral component of chlorophyll breakdown. It catalyzes the demethylation of fluorescent chlorophyll catabolite (FCC) and pheophorbide in vitro, and specifically demethylates FCC in vivo. Here we report the crystal structure of MES16 from Arabidopsis thaliana at 2.8 angstrom resolution. The structure confirm that MES16 is a member of the alpha/beta-hydrolase superfamily with Ser-87, His-239, and Asp-211 as the catalytic triad. Our biochemical studies reveal that MES16 has esterase activity with methyl-indole acetic acid as the substrate, and the catalytically essential role of Ser-87 has been demonstrated. (C) 2016 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Biophysics
电子版国际标准刊号	1090-2104
出版地	SAN DIEGO
WOS关键词	CHLOROPHYLL BREAKDOWN ; HYDROXYNITRILE LYASE ; CATABOLITES ; DEGRADATION ; ENZYME ; BIOSYNTHESIS ; ESTERASE ; CLONING ; LEAVES ; PATH
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000376810600034
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31300634]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/24668]
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynthesis Res Ctr, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 101048, Peoples R China
推荐引用方式 GB/T 7714	Li, Hongmei,Pu, Hua. Crystal structure of methylesterase family member 16 (MES16) from Arabidopsis thaliana[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2016,474(1).
APA	Li, Hongmei,&Pu, Hua.(2016).Crystal structure of methylesterase family member 16 (MES16) from Arabidopsis thaliana.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,474(1).
MLA	Li, Hongmei,et al."Crystal structure of methylesterase family member 16 (MES16) from Arabidopsis thaliana".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 474.1(2016).