Crystal structure of glutamate-1-semialdehyde-2,1-aminomutase from Arabidopsis thaliana

doi:10.1107/S2053230X16007263

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	Crystal structure of glutamate-1-semialdehyde-2,1-aminomutase from Arabidopsis thaliana
	Song, Yingxian; Pu, Hua; Jiang, Tian; Zhang, Lixin; Ouyang, Min
刊名	ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
	2016
卷号	72
关键词	X-ray crystallography asymmetry pyridoxamine 5 '-phosphate pyridoxal 5 '-phosphate gating loop glutamate-1-semialdehyde-2,1-aminomutase Arabidopsis thaliana
DOI	10.1107/S2053230X16007263
文献子类	Article
英文摘要	Glutamate-1-semialdehyde-2,1-aminomutase (GSAM) catalyzes the isomerization of glutamate-1-semialdehyde (GSA) to 5-aminolevulinate (ALA) and is distributed in archaea, most bacteria and plants. Although structures of GSAM from archaea and bacteria have been resolved, a GSAM structure from a higher plant is not available, preventing further structure-function analysis. Here, the structure of GSAM from Arabidopsis thaliana (AtGSA1) obtained by X-ray crystallography is reported at 1.25 angstrom resolution. AtGSA1 forms an asymmetric dimer and displays asymmetry in cofactor binding as well as in the gating-loop orientation, which is consistent with previously reported Synechococcus GSAM structures. While one monomer binds PMP with the gating loop fixed in the open state, the other monomer binds either PMP or PLP and the gating loop is ready to close. The data also reveal the mobility of residues Gly163, Ser164 and Gly165, which are important for reorientation of the gating loop. Furthermore, the asymmetry of the AtGSA1 structure supports the previously proposed negative cooperativity between monomers of GSAM.
学科主题	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
电子版国际标准刊号	2053-230X
出版地	CHESTER
WOS关键词	GLUTAMYL-TRANSFER-RNA ; NEGATIVE COOPERATIVITY ; 1-SEMIALDEHYDE AMINOTRANSFERASE ; SEMIALDEHYDE AMINOTRANSFERASE ; CHLOROPHYLL BIOSYNTHESIS ; ENZYME ; 2,1-AMINOMUTASE ; SYNECHOCOCCUS ; MECHANISM ; REDUCTASE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	INT UNION CRYSTALLOGRAPHY
WOS记录号	WOS:000380288600005
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471267]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/24665]
专题	中科院光生物学重点实验室
作者单位	Chinese Acad Sci, Photosynth Res Ctr, Key Lab Photobiol, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Song, Yingxian,Pu, Hua,Jiang, Tian,et al. Crystal structure of glutamate-1-semialdehyde-2,1-aminomutase from Arabidopsis thaliana[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2016,72.
APA	Song, Yingxian,Pu, Hua,Jiang, Tian,Zhang, Lixin,&Ouyang, Min.(2016).Crystal structure of glutamate-1-semialdehyde-2,1-aminomutase from Arabidopsis thaliana.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,72.
MLA	Song, Yingxian,et al."Crystal structure of glutamate-1-semialdehyde-2,1-aminomutase from Arabidopsis thaliana".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 72(2016).