Flavone synthases from Lonicera japonica and L. macranthoides reveal differential flavone accumulation | |
Wu, Jie; Wang, Xiao-Chen; Liu, Yang; Du, Hui; Shu, Qing-Yan; Su, Shang; Wang, Li-Jin; Li, Shan-Shan; Wang, Liang-Sheng | |
刊名 | SCIENTIFIC REPORTS |
2016 | |
卷号 | 6 |
ISSN号 | 2045-2322 |
DOI | 10.1038/srep19245 |
文献子类 | Article |
英文摘要 | Flavones are important secondary metabolites found in many plants. In Lonicera species, flavones contribute both physiological and pharmaceutical properties. However, flavone synthase (FNS), the key enzyme responsible for flavone biosynthesis, has not yet been characterized in Lonicera species. In this study, FNSII genes were identified from Lonicera japonica Thunb. and L. macranthoides Hand. Mazz. In the presence of NADPH, the recombinant cytochrome P450 proteins encoded by LjFNSII-1.1, LjFNSII-2.1, and LmFNSII-1.1 converted eriodictyol, naringenin, and liquiritigenin to the corresponding flavones directly. The different catalytic properties between LjFNSII-2.1 and LjFNSII-1.1 were caused by a single amino acid substitution at position 242 (glutamic acid to lysine). A methionine at position 206 and a leucine at position 381 contributed considerably to the high catalytic activity of LjFNSII-1.1. In addition, LjFNSII-1.1&2.1 and LmFNSII-1.1 also biosynthesize flavones that were further modified by O-glycosylation in transgenic tobacco. The expression levels of the FNSII genes were consistent with flavone accumulation patterns in flower buds. Our findings suggested that the weak catalytic activity of LmFNSII-1.1 and the relatively low expression of LmFNSII-1.1 in flowers might be responsible for the low levels of flavone accumulation in flower buds of L. macranthoides. |
学科主题 | Multidisciplinary Sciences |
出版地 | LONDON |
WOS关键词 | CYTOCHROME-P450 GENES ; INDUCIBLE FLAVONE ; IDENTIFICATION ; EXPRESSION ; BIOSYNTHESIS ; CLONING ; MAIZE ; GLYCOSYLATION ; 2-HYDROXYLASE ; LUTEOLIN |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
语种 | 英语 |
出版者 | NATURE PUBLISHING GROUP |
WOS记录号 | WOS:000368108000002 |
资助机构 | National Science and Technology Basic Project of China [2014FY110100] |
内容类型 | 期刊论文 |
源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/24737] |
专题 | 中科院植物分子生理学重点实验室 |
作者单位 | 1.Su, Shang 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Wang, Xiao-Chen 5.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Key Lab Plant Resources, Beijing 100093, Peoples R China 6.Li, Shan-Shan 7.Wang, Li-Jin 8.Shu, Qing-Yan 9.Du, Hui 10.Liu, Yang |
推荐引用方式 GB/T 7714 | Wu, Jie,Wang, Xiao-Chen,Liu, Yang,et al. Flavone synthases from Lonicera japonica and L. macranthoides reveal differential flavone accumulation[J]. SCIENTIFIC REPORTS,2016,6. |
APA | Wu, Jie.,Wang, Xiao-Chen.,Liu, Yang.,Du, Hui.,Shu, Qing-Yan.,...&Wang, Liang-Sheng.(2016).Flavone synthases from Lonicera japonica and L. macranthoides reveal differential flavone accumulation.SCIENTIFIC REPORTS,6. |
MLA | Wu, Jie,et al."Flavone synthases from Lonicera japonica and L. macranthoides reveal differential flavone accumulation".SCIENTIFIC REPORTS 6(2016). |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论