Role of UDP-Glucuronic Acid Decarboxylase in Xylan Biosynthesis in Arabidopsis

doi:10.1016/j.molp.2016.04.013

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	Role of UDP-Glucuronic Acid Decarboxylase in Xylan Biosynthesis in Arabidopsis
	Kuang, Beiqing ; Zhao, Xianhai ; Zhou, Chun ; Zeng, Wei ; Ren, Junli 4; Ebert, Berit ; Beahan, Cherie T.; Deng, Xiaomei ; Zeng, Qingyin 5; Zhou, Gongke 9
刊名	MOLECULAR PLANT
	2016
卷号	9 期号:8
关键词	UDP-Xylose xylan UDP-Glucuronic acid decarboxylase localization
ISSN号	1674-2052
DOI	10.1016/j.molp.2016.04.013
文献子类	Article
英文摘要	UDP-xylose (UDP-Xyl) is the Xyl donor used in the synthesis of major plant cell-wall polysaccharides such as xylan (as a backbone-chain monosaccharide) and xyloglucan (as a branching monosaccharide). The biosynthesis of UDP-Xyl from UDP-glucuronic acid (UDP-GlcA) is irreversibly catalyzed by UDPglucuronic acid decarboxylase (UXS). Until now, little has been known about the physiological roles of UXS in plants. Here, we report that AtUXS1, AtUXS2, and AtUXS4 are located in the Golgi apparatus whereas AtUXS3, AtUXS5, and AtUXS6 are located in the cytosol. Although all six single AtUXS T-DNA mutants and the uxs1 usx2 uxs4 triple mutant show no obvious phenotype, the uxs3 uxs5 uxs6 triple mutant has an irregular xylem phenotype. Monosaccharide analysis showed that Xyl levels decreased in uxs3 uxs5 uxs6 and linkage analysis confirmed that the xylan content in uxs3 xus5 uxs6 declined, indicating that UDP-Xyl from cytosol AtUXS participates in xylan synthesis. Gel-permeation chromatography showed that the molecular weight of non-cellulosic polysaccharides in the triple mutants, mainly composed of xylans, is lower than that in the wild type, suggesting an effect on the elongation of the xylan backbone. Upon saccharification treatment stems of the uxs3 uxs5 uxs6 triple mutants released monosaccharides with a higher efficiency than those of the wild type. Taken together, our results indicate that the cytosol UXS plays a more important role than the Golgi-localized UXS in xylan biosynthesis.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences
电子版国际标准刊号	1752-9867
出版地	CAMBRIDGE
WOS关键词	PLANT-CELL WALLS ; D-XYLOSE SYNTHASE ; RHAMNOGALACTURONAN-II ; MOLECULAR-CLONING ; FUNCTIONAL CLONING ; GOLGI MEMBRANES ; XYLOSYLTRANSFERASE ; EXPRESSION ; GENES ; XYLOGLUCAN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	CELL PRESS
WOS记录号	WOS:000386584900004
资助机构	Science and Technology Planning Project of Guangdong Province [2015A050502045] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31170165, 31270594] ; Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2013AA102705, 2013BAD22B01] ; Guangdong Natural Science foundationNational Natural Science Foundation of Guangdong Province [S2013010011988] ; Taishan Scholar Program of Shandong ; ARC Center of Excellence in Plant Cell Walls, AustraliaAustralian Research Council [CE110001007]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/24722]
专题	中科院植物分子生理学重点实验室
作者单位	1.Guangdong Prov Res Ctr Woody Forage Engn Technol, Guangzhou 510642, Guangdong, Peoples R China 2.South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou 510642, Guangdong, Peoples R China 3.South China Agr Univ, Coll Forestry & Landscape Architecture, Guangdong Key Lab Innovat Dev & Utilizat Forest P, Guangzhou 510642, Guangdong, Peoples R China 4.Univ Melbourne, Sch BioSci, ARC Ctr Excellence Plant Cell Walls, Parkville, Vic 3010, Australia 5.South China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou 510640, Guangdong, Peoples R China 6.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 7.Heazlewood, Joshua L.] Lawrence Berkeley Natl Lab, Joint BioEnergy Inst, Berkeley, CA 94720 USA 8.Heazlewood, Joshua L.] Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA 94720 USA 9.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Kuang, Beiqing,Zhao, Xianhai,Zhou, Chun,et al. Role of UDP-Glucuronic Acid Decarboxylase in Xylan Biosynthesis in Arabidopsis[J]. MOLECULAR PLANT,2016,9(8).
APA	Kuang, Beiqing.,Zhao, Xianhai.,Zhou, Chun.,Zeng, Wei.,Ren, Junli.,...&Wu, Ai-Min.(2016).Role of UDP-Glucuronic Acid Decarboxylase in Xylan Biosynthesis in Arabidopsis.MOLECULAR PLANT,9(8).
MLA	Kuang, Beiqing,et al."Role of UDP-Glucuronic Acid Decarboxylase in Xylan Biosynthesis in Arabidopsis".MOLECULAR PLANT 9.8(2016).