Comparative Proteomics Reveals the Potential Targets of BcNoxR, a Putative Regulatory Subunit of NADPH Oxidase of Botrytis cinerea

doi:10.1094/MPMI-11-16-0227-R

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	Comparative Proteomics Reveals the Potential Targets of BcNoxR, a Putative Regulatory Subunit of NADPH Oxidase of Botrytis cinerea
	Li, Hua 1; Zhang, Zhanquan ; He, Chang 1; Qin, Guozheng ; Tian, Shiping 1
刊名	MOLECULAR PLANT-MICROBE INTERACTIONS
	2016
卷号	29 期号:12
ISSN号	0894-0282
DOI	10.1094/MPMI-11-16-0227-R
文献子类	Article
英文摘要	The NADPH oxidase (NOX) complex has been shown to play a crucial role in stress response and in the virulence of various fungal pathogens. The underlying molecular mechanisms of NOX, however, remain largely unknown. In the present study, a comparative proteomic analysis compared changes in protein abundance in wild-type Bonytis cinerea and Delta bcnoxR mutants in which the regulatory subunit of NOX was deleted. The Delta bcnoxR mutants exhibited reduced growth, sporulation, and impaired virulence. A total of 60 proteins, representing 49 individual genes, were identified in Delta bcnoxR mutants that exhibited significant differences in abundance relative to wild-type. Reverse transcription-quantitative polymerase chain reaction analysis demonstrated that the differences in transcript levels for 36 of the genes encoding the identified proteins were in agreement with the proteomic analysis, while the remainder exhibited reverse levels. Functional analysis of four proteins that decreased abundance in the Delta banoxR mutants indicated that 6-phosphogluconate dehydrogenase (BcPGD) played a role in the growth and sporulation of B. cinerea. The Delta bcpgd mutants also displayed impaired virulence on various hosts, such as apple, strawberry, and tomato fruit. These results suggest that NOX can influence the expression of BcPGD, which has an impact on growth, sporulation, and virulence of B. cinerea.
学科主题	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Plant Sciences
电子版国际标准刊号	1943-7706
出版地	ST PAUL
WOS关键词	L-PROLINE ACCUMULATION ; VON-WILLEBRAND-FACTOR ; REACTIVE OXYGEN ; SACCHAROMYCES-CEREVISIAE ; ASPERGILLUS-NIDULANS ; FUNCTIONAL-ANALYSIS ; FREEZE TOLERANCE ; VIRULENCE ; EXPRESSION ; FUNGUS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	AMER PHYTOPATHOLOGICAL SOC
WOS记录号	WOS:000393014200007
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31530057, 31172004]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/24714]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Hua,Zhang, Zhanquan,He, Chang,et al. Comparative Proteomics Reveals the Potential Targets of BcNoxR, a Putative Regulatory Subunit of NADPH Oxidase of Botrytis cinerea[J]. MOLECULAR PLANT-MICROBE INTERACTIONS,2016,29(12).
APA	Li, Hua,Zhang, Zhanquan,He, Chang,Qin, Guozheng,&Tian, Shiping.(2016).Comparative Proteomics Reveals the Potential Targets of BcNoxR, a Putative Regulatory Subunit of NADPH Oxidase of Botrytis cinerea.MOLECULAR PLANT-MICROBE INTERACTIONS,29(12).
MLA	Li, Hua,et al."Comparative Proteomics Reveals the Potential Targets of BcNoxR, a Putative Regulatory Subunit of NADPH Oxidase of Botrytis cinerea".MOLECULAR PLANT-MICROBE INTERACTIONS 29.12(2016).