Proteomic Dissection of Endosperm Starch Granule Associated Proteins Reveals a Network Coordinating Starch Biosynthesis and Amino Acid Metabolism and Glycolysis in Rice Endosperms

doi:10.3389/fpls.2016.00707

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院植物分子生理学重点实验室

	Proteomic Dissection of Endosperm Starch Granule Associated Proteins Reveals a Network Coordinating Starch Biosynthesis and Amino Acid Metabolism and Glycolysis in Rice Endosperms
	Yu, Huatao 1; Wang, Tai
刊名	FRONTIERS IN PLANT SCIENCE
	2016
卷号	7
关键词	starch granules starch granules-associated proteins endosperm starch biosynthesis rice
ISSN号	1664-462X
DOI	10.3389/fpls.2016.00707
文献子类	Article
英文摘要	Starch biosynthesis and starch granule packaging in cereal endosperms involve a coordinated action of starch biosynthesis enzymes and coordination with other metabolisms. Because directly binding to starch granules, starch granule-associated proteins (SGAPs) are essential to understand the underlying mechanisms, however the information on SGAPs remains largely unknown. Here, we dissected developmentally changed SGAPs from developing rice endosperms from 10 to 20 days after flowering (DAF). Starch granule packaging was not completed at 10 DAF, and was finished in the central endosperm at 15 DAF and in the whole endosperm at 20 DAF. Proteomic analysis with two-dimensional differential in-gel electrophoresis and mass spectrometry revealed 115 developmentally changed SGAPs, representing 37 unique proteins. 65% of the unique proteins had isoforms. 39% of the identified SGAPs were involved in starch biosynthesis with main functions in polyglucan elongation and granule structure trimming. Almost all proteins involved in starch biosynthesis, amino acid biosynthesis, glycolysis, protein folding, and PPDK pathways increased abundance as the endosperm developed, and were predicted in an interaction network. The network represents an important mechanism to orchestrate carbon partitioning among starch biosynthesis, amino acid biosynthesis and glycolysis for efficient starch and protein storage. These results provide novel insights into mechanisms of starch biosynthesis and its coordination with amino acid metabolisms and glycolysis in cereal endosperms.
学科主题	Plant Sciences
出版地	LAUSANNE
WOS关键词	TRITICUM-AESTIVUM L. ; WHEAT ; AMYLOPLASTS ; BARLEY ; MAIZE ; ISOAMYLASE ; ENZYMES ; EXTRACTION ; INITIATION ; COMPLEXES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000376745000001
资助机构	Chinese Ministry of Science and TechnologyMinistry of Science and Technology, China [2013CBA01402, 2012CB910504]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/24705]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 2.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Yu, Huatao,Wang, Tai. Proteomic Dissection of Endosperm Starch Granule Associated Proteins Reveals a Network Coordinating Starch Biosynthesis and Amino Acid Metabolism and Glycolysis in Rice Endosperms[J]. FRONTIERS IN PLANT SCIENCE,2016,7.
APA	Yu, Huatao,&Wang, Tai.(2016).Proteomic Dissection of Endosperm Starch Granule Associated Proteins Reveals a Network Coordinating Starch Biosynthesis and Amino Acid Metabolism and Glycolysis in Rice Endosperms.FRONTIERS IN PLANT SCIENCE,7.
MLA	Yu, Huatao,et al."Proteomic Dissection of Endosperm Starch Granule Associated Proteins Reveals a Network Coordinating Starch Biosynthesis and Amino Acid Metabolism and Glycolysis in Rice Endosperms".FRONTIERS IN PLANT SCIENCE 7(2016).