The role of Arabidopsis Actin-Related Protein 3 in amyloplast sedimentation and polar auxin transport in root gravitropism

doi:10.1093/jxb/erw294

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	The role of Arabidopsis Actin-Related Protein 3 in amyloplast sedimentation and polar auxin transport in root gravitropism
	Zou, Jun-Jie ; Zheng, Zhong-Yu ; Xue, Shan 1; Li, Han-Hai ; Wang, Yu-Ren ; Le, Jie
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2016
卷号	67 期号:18
关键词	Amyloplast arabidopsis ARP2/3 auxin gravitropism PINs
ISSN号	0022-0957
DOI	10.1093/jxb/erw294
文献子类	Article
英文摘要	Arabidopsis actin-related protein ARP3 plays a role in amyloplast sedimentation and polar auxin redistribution during root gravitropism.Gravitropism is vital for shaping directional plant growth in response to the forces of gravity. Signals perceived in the gravity-sensing cells can be converted into biochemical signals and transmitted. Sedimentation of amyloplasts in the columella cells triggers asymmetric auxin redistribution in root tips, leading to downward root growth. The actin cytoskeleton is thought to play an important role in root gravitropism, although the molecular mechanism has not been resolved. DISTORTED1 (DIS1) encodes the ARP3 subunit of the Arabidopsis Actin-Related Protein 2/3 (ARP2/3) complex, and the ARP3/DIS1 mutant dis1-1 showed delayed root curvature after gravity stimulation. Microrheological analysis revealed that the high apparent viscosity within dis1-1 central columella cells is closely associated with abnormal movement trajectories of amyloplasts. Analysis using a sensitive auxin input reporter DII-VENUS showed that asymmetric auxin redistribution was reduced in the root tips of dis1-1, and the actin-disrupting drug Latrunculin B increased the asymmetric auxin redistribution. An uptake assay using the membrane-selective dye FM4-64 indicated that endocytosis was decelerated in dis1-1 root epidermal cells. Treatment and wash-out with Brefeldin A, which inhibits protein transport from the endoplasmic reticulum to the Golgi apparatus, showed that cycling of the auxin-transporter PIN-FORMED (PIN) proteins to the plasma membrane was also suppressed in dis1-1 roots. The results reveal that ARP3/DIS1 acts in root gravitropism by affecting amyloplast sedimentation and PIN-mediated polar auxin transport through regulation of PIN protein trafficking.
学科主题	Plant Sciences
电子版国际标准刊号	1460-2431
出版地	OXFORD
WOS关键词	ARG1 ALTERED-RESPONSE ; ARP2/3 COMPLEX ; COLUMELLA CELLS ; EFFLUX CARRIER ; F-ACTIN ; PLANT GRAVITROPISM ; CYTOSKELETON ; DYNAMICS ; PIN2 ; THALIANA
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000386067000006
资助机构	National Basic Research Program of ChinaNational Basic Research Program of China [2011CB710902, 2011CB710901] ; Strategic Pioneer Program on Space Science, Chinese Academy of Sciences [XDA04079100]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/24694]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Zou, Jun-Jie,Zheng, Zhong-Yu,Xue, Shan,et al. The role of Arabidopsis Actin-Related Protein 3 in amyloplast sedimentation and polar auxin transport in root gravitropism[J]. JOURNAL OF EXPERIMENTAL BOTANY,2016,67(18).
APA	Zou, Jun-Jie,Zheng, Zhong-Yu,Xue, Shan,Li, Han-Hai,Wang, Yu-Ren,&Le, Jie.(2016).The role of Arabidopsis Actin-Related Protein 3 in amyloplast sedimentation and polar auxin transport in root gravitropism.JOURNAL OF EXPERIMENTAL BOTANY,67(18).
MLA	Zou, Jun-Jie,et al."The role of Arabidopsis Actin-Related Protein 3 in amyloplast sedimentation and polar auxin transport in root gravitropism".JOURNAL OF EXPERIMENTAL BOTANY 67.18(2016).