Metagenomic Analysis Reveals Symbiotic Relationship among Bacteria in Microcystis-Dominated Community

doi:10.3389/fmicb.2016.00056

CORC > 植物研究所 > 中国科学院植物研究所 > 系统与进化植物学国家重点实验室

	Metagenomic Analysis Reveals Symbiotic Relationship among Bacteria in Microcystis-Dominated Community
	Xie, Meili 2; Ren, Minglei 2; Yang, Chen 2; Yi, Haisi 2; Li, Zhe 3; Li, Tao ; Zhao, Jindong 1
刊名	FRONTIERS IN MICROBIOLOGY
	2016
卷号	7
关键词	metagenome binning Microcystis bloom symbiosis
ISSN号	1664-302X
DOI	10.3389/fmicb.2016.00056
文献子类	Article
英文摘要	Microcystis bloom, a cyanobacterial mass occurrence often found in eutrophicated water bodies, is one of the most serious threats to freshwater ecosystems worldwide. In nature, Microcystis forms aggregates or colonies that contain heterotrophic bacteria. The Microcystis-bacteria colonies were persistent even when they were maintained in lab culture for a long period. The relationship between Microcystis and the associated bacteria was investigated by a metagenomic approach in this study. We developed a visualization -guided method of binning for genome assembly after total colony DNA sequencing. We found that the method was effective in grouping sequences and it did not require reference genome sequence. Individual genomes of the colony bacteria were obtained and they provided valuable insights into microbial community structures. Analysis of metabolic pathways based on these genomes revealed that while all heterotrophic bacteria were dependent upon Microcystis for carbon and energy, Vitamin B12 biosynthesis, which is required for growth by Microcystis, was accomplished in a cooperative fashion among the bacteria. Our analysis also suggests that individual bacteria in the colony community contributed a complete pathway for degradation of benzoate, which is inhibitory to the cyanobacterial growth, and its ecological implication for Microcystis bloom is discussed.
学科主题	Microbiology
出版地	LAUSANNE
WOS关键词	SEQUENCES ; LIPOPOLYSACCHARIDE ; BLOOMS ; LAKE ; VITAMIN-B-12 ; METABOLISM ; AERUGINOSA ; ALGORITHM ; COVERAGE ; GENOMES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000369113000002
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31000113] ; National High-Technology Research and Development Program of China (863 Program)National High Technology Research and Development Program of China [2009AA02Z30] ; Autonomous Projects of the State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ31, 2011FBZ32]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/24417]
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, State Key Lab Systemat & Evolutionary Bot, Inst Bot, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Xie, Meili,Ren, Minglei,Yang, Chen,et al. Metagenomic Analysis Reveals Symbiotic Relationship among Bacteria in Microcystis-Dominated Community[J]. FRONTIERS IN MICROBIOLOGY,2016,7.
APA	Xie, Meili.,Ren, Minglei.,Yang, Chen.,Yi, Haisi.,Li, Zhe.,...&Zhao, Jindong.(2016).Metagenomic Analysis Reveals Symbiotic Relationship among Bacteria in Microcystis-Dominated Community.FRONTIERS IN MICROBIOLOGY,7.
MLA	Xie, Meili,et al."Metagenomic Analysis Reveals Symbiotic Relationship among Bacteria in Microcystis-Dominated Community".FRONTIERS IN MICROBIOLOGY 7(2016).