State of the phycobilisome determines effective absorption cross-section of Photosystem II in Synechocystis sp. PCC 6803

doi:10.1016/j.bbabio.2021.148494

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	State of the phycobilisome determines effective absorption cross-section of Photosystem II in Synechocystis sp. PCC 6803
	Protasova, Elena A.7; Antal, Taras K.7; Zlenko, Dmitry, V 7; Elanskaya, Irina, V 6; Lukashev, Evgeny P.7; Friedrich, Thomas 5; Mironov, Kirill S.4; Sluchanko, Nikolai N.3; Ge, Baosheng 2; Qin, Song 1,2
刊名	BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
	2021-12-01
卷号	1862 期号:12 页码:11
关键词	Nitrogen starvation Phycobiliproteins Photosystem II Orange Carotenoid Protein
ISSN号	0005-2728
DOI	10.1016/j.bbabio.2021.148494
通讯作者	Protasova, Elena A.(123protasovaelen@gmail.com) ; Qin, Song(sqin@yic.ac.cn)
英文摘要	Quenching of excess excitation energy is necessary for the photoprotection of light-harvesting complexes. In cyanobacteria, quenching of phycobilisome (PBS) excitation energy is induced by the Orange Carotenoid Protein (OCP), which becomes photoactivated under high light conditions. A decrease in energy transfer efficiency from the PBSs to the reaction centers decreases photosystem II (PS II) activity. However, quantitative analysis of OCPinduced photoprotection in vivo is complicated by similar effects of both photochemical and non-photochemical quenching on the quantum yield of the PBS fluorescence overlapping with the emission of chlorophyll. In the present study, we have analyzed chlorophyll a fluorescence induction to estimate the effective cross-section of PS II and compared the effects of reversible OCP-dependent quenching of PBS fluorescence with reduction of PBS content upon nitrogen starvation or mutations of key PBS components. This approach allowed us to estimate the dependency of the rate constant of PS II primary electron acceptor reduction on the amount of PBSs in the cell. We found that OCP-dependent quenching triggered by blue light affects approximately half of PBSs coupled to PS II, indicating that under normal conditions, the concentration of OCP is not sufficient for quenching of all PBSs coupled to PS II.
WOS关键词	ORANGE CAROTENOID PROTEIN ; SP PCC6803 ; NITROGEN-STARVATION ; REACTION CENTERS ; ENERGY-TRANSFER ; CHLOROPHYLL-A ; CYANOBACTERIUM ; FLUORESCENCE ; MUTANT ; CORE
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
语种	英语
WOS记录号	WOS:000712537100004
资助机构	Russian Science Foundation ; German Research Foundation DFG ; National Natural Science Foundation of China ; Program of the Russian Ministry of Science and Higher Education
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/30720]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者	Protasova, Elena A.; Qin, Song
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.China Univ Petr Huadong, Coll Chem Engn, Qingdao 266580, Peoples R China 3.Russian Acad Sci, Fed Res Ctr Biotechnol, AN Bach Inst Biochem, Moscow 119071, Russia 4.Russian Acad Sci, Timiryazev Inst Plant Physiol, Moscow 127276, Russia 5.Tech Univ Berlin, Inst Chem, D-10623 Berlin, Germany 6.Lomonosov Moscow State Univ, Fac Biol, Dept Genet, Moscow 119991, Russia 7.Lomonosov Moscow State Univ, Fac Biol, Dept Biophys, Moscow 119991, Russia
推荐引用方式 GB/T 7714	Protasova, Elena A.,Antal, Taras K.,Zlenko, Dmitry, V,et al. State of the phycobilisome determines effective absorption cross-section of Photosystem II in Synechocystis sp. PCC 6803[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,2021,1862(12):11.
APA	Protasova, Elena A..,Antal, Taras K..,Zlenko, Dmitry, V.,Elanskaya, Irina, V.,Lukashev, Evgeny P..,...&Maksimov, Eugene G..(2021).State of the phycobilisome determines effective absorption cross-section of Photosystem II in Synechocystis sp. PCC 6803.BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,1862(12),11.
MLA	Protasova, Elena A.,et al."State of the phycobilisome determines effective absorption cross-section of Photosystem II in Synechocystis sp. PCC 6803".BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1862.12(2021):11.