A Peptide from Budding Yeast GAPDH Serves as a Promising Antifungal against Cryptococcus neoformans

doi:10.1128/spectrum.00826-21

	A Peptide from Budding Yeast GAPDH Serves as a Promising Antifungal against Cryptococcus neoformans
	Zhang, Yang 1,2; Zhou, Liyan 2,3,6; Liu, Yan 2,3; Zhao, Xi 2,3; Lian, Xianqiang 2,3; Zhang, Jie 2; Zhao, De 2,3; Wang, Yujuan 4; Zhong, Jin 2; Wang, Junfeng 4
刊名	MICROBIOLOGY SPECTRUM
	2022-02-01
卷号	10
关键词	antifungal peptide GAPDH Cryptococcus neoformans apoptosis
ISSN号	2165-0497
DOI	10.1128/spectrum.00826-21
通讯作者	Zhou, Liyan() ; Wang, Linqi(wanglq@im.ac.cn) ; Fu, Yu, V(fuyu@im.ac.cn)
英文摘要	Infection of Cryptococcus neoformans is one of the leading causes of morbidity and mortality, particularly among immunocompromised patients. However, currently available drugs for the treatment of C. neoformans infection are minimal. Here, we report SP1, a peptide derived from glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of Saccharomyces cerevisiae, efficiently kills C. neoformans and Cryptococcus gattii. SP1 causes damages to the capsule. Unlike many antimicrobial peptides, SP1 does not form pores on the cell membrane of C. neoformans. It interacts with membrane ergosterol and enters vacuole possibly through membrane trafficking. C. neoformans treated with SP1 show the apoptotic phenotypes such as imbalance of calcium ion homeostasis, reactive oxygen increment, phosphatidylserine exposure, and nuclear fragmentation. Our data imply that SP1 has the potential to be developed into a treatment option for cryptococcosis. IMPORTANCE Cryptococcus neoformans and Cryptococcus gattii can cause cryptococcosis, which has a high mortality rate. To treat the disease, amphotericin B and fluconazole are often used in clinic. However, amphotericin B has rather high renal toxicity, and tolerance to these drugs are quicky developed. The peptide SP1 derived from baker's yeast GAPDH shows antifungal function to kill Cryptococcus neoformans and Cryptococcus gattii efficiently with a high specificity, even for the drug-resistant strains. Our data demonstrate that SP1 induces the apoptosis-like death of Cryptococcus neoformans at low concentrations. The finding of this peptide may shed light on a new direction to treat cryptococcosis.
资助项目	National Natural Science Foundation of China[31970553] ; National Natural Science Foundation of China[31571288] ; National Natural Science Foundation of China[52091541] ; Chinese Academy of Sciences[GQRC-19-18] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA17010503] ; Senior User Project of RV KEXUE[KEXUE2019GZ05] ; Royal Society[NA140085]
WOS关键词	PROTEIN SECONDARY STRUCTURE ; CIRCULAR-DICHROISM SPECTROSCOPY ; ANTIMICROBIAL PEPTIDES ; SWISS-MODEL ; HIGH POTENCY ; MEMBRANE ; DIVERSITY ; VIRULENCE ; CA2+
WOS研究方向	Microbiology
语种	英语
出版者	AMER SOC MICROBIOLOGY
WOS记录号	WOS:000766015800024
资助机构	National Natural Science Foundation of China ; Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Senior User Project of RV KEXUE ; Royal Society
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/128667]
专题	中国科学院合肥物质科学研究院
通讯作者	Zhou, Liyan; Wang, Linqi; Fu, Yu, V
作者单位	1.Univ Sci & Technol China, Sch Life Sci, Div Life Sci & Med, Hefei, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Savaid Med Sch, Beijing, Peoples R China 4.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Hefei, Peoples R China 5.Capital Med Univ, Beijing Obstet & Gynecol Hosp, Dept Lab Med, Beijing, Peoples R China 6.Guangxi Med Univ, Dept Clin Lab, Affiliated Hosp 2, Nanning, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yang,Zhou, Liyan,Liu, Yan,et al. A Peptide from Budding Yeast GAPDH Serves as a Promising Antifungal against Cryptococcus neoformans[J]. MICROBIOLOGY SPECTRUM,2022,10.
APA	Zhang, Yang.,Zhou, Liyan.,Liu, Yan.,Zhao, Xi.,Lian, Xianqiang.,...&Fu, Yu, V.(2022).A Peptide from Budding Yeast GAPDH Serves as a Promising Antifungal against Cryptococcus neoformans.MICROBIOLOGY SPECTRUM,10.
MLA	Zhang, Yang,et al."A Peptide from Budding Yeast GAPDH Serves as a Promising Antifungal against Cryptococcus neoformans".MICROBIOLOGY SPECTRUM 10(2022).