Identification of Long Noncoding RNAs Associated With the Clinicopathological Features of Papillary Thyroid Carcinoma Complicated With Hashimoto's Thyroiditis

doi:10.3389/fonc.2022.766016

	Identification of Long Noncoding RNAs Associated With the Clinicopathological Features of Papillary Thyroid Carcinoma Complicated With Hashimoto's Thyroiditis
	Zhang, Yu 1; Lu, Kai-Ning 1; Ding, Jin-Wang 2; Peng, You 1; Pan, Gang 1; Teng, Li-Song 3; Luo, Ding-Cun 1
刊名	FRONTIERS IN ONCOLOGY
	2022-03-11
卷号	12
关键词	Hashimoto's thyroiditis papillary thyroid carcinoma clinicopathological features long noncoding RNA microarray
ISSN号	2234-943X
DOI	10.3389/fonc.2022.766016
通讯作者	Teng, Li-Song(lsteng@zju.edu.cn) ; Luo, Ding-Cun(ldc65@163.com)
英文摘要	Long noncoding RNAs (lncRNAs) play a significant role in cancer biology. This study aimed to determine the roles of lncRNAs in establishing the differences in clinical features between patients with papillary thyroid carcinoma (PTC) without Hashimoto's thyroiditis (HT) and patients with PTC and HT. In the present study, we detected the differentially expressed lncRNAs between tumor tissues of patients with PTC with or without HT through lncRNA microarrays. The data were verified and analyzed through qRT-PCR, cell viability, cell cycle and bioinformatics analyses. We found that 1031 lncRNAs and 1338 mRNAs were abnormally expressed in 5 tissue samples of PTC complicated with HT [PTC/HT (+)] compared with 5 samples of PTC without HT [PTC/HT (-)]. Gene Ontology and pathway analyses of the mRNAs suggested that several biological processes and pathways, particularly immune system processes, were induced in the PTC/HT (+) tissues. Twenty lncRNAs were verified in 31 PTC/HT (+) and 64 PTC/HT (-) specimens by qRT-PCR, and the results were consistent with the microarray data. Specifically, ENST00000452578, a downregulated lncRNA in PTC/HT(+), was negatively correlated with the tumor size. Cell viability assays revealed that ENST00000452578 could inhibit cell proliferation. Our results indicate that lncRNAs and mRNAs play an important role in establishing the different clinical characteristics between patients with PTC/HT(+) and patients with PTC/HT(-), and might provide new insights from the perspective of RNA for obtaining a further understanding of the clinical features related to PTC with HT.
资助项目	Key Project of Medical Scientific and Technology Program in Hangzhou[2013Z04] ; Project of Medical Scientific and Technology Program in Hangzhou[A20200432] ; Medical and Health Research Program of Zhejiang Province[2019RC240] ; Key Project of Scientific and Technology of Hangzhou Health Commission[OO20190490]
WOS关键词	CHRONIC LYMPHOCYTIC THYROIDITIS ; CANCER STATISTICS
WOS研究方向	Oncology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000778433700001
资助机构	Key Project of Medical Scientific and Technology Program in Hangzhou ; Project of Medical Scientific and Technology Program in Hangzhou ; Medical and Health Research Program of Zhejiang Province ; Key Project of Scientific and Technology of Hangzhou Health Commission
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/128489]
专题	中国科学院合肥物质科学研究院
通讯作者	Teng, Li-Song; Luo, Ding-Cun
作者单位	1.Zhejiang Univ, Affiliated Hangzhou Peoples Hosp 1, Dept Oncol Surg, Sch Med, Hangzhou, Peoples R China 2.Univ Chinese Acad Sci, Dept Head & Neck Surg, Canc Hosp, Hangzhou, Peoples R China 3.Zhejiang Univ, Affiliated Hosp 1, Canc Ctr, Sch Med, Hangzhou, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yu,Lu, Kai-Ning,Ding, Jin-Wang,et al. Identification of Long Noncoding RNAs Associated With the Clinicopathological Features of Papillary Thyroid Carcinoma Complicated With Hashimoto's Thyroiditis[J]. FRONTIERS IN ONCOLOGY,2022,12.
APA	Zhang, Yu.,Lu, Kai-Ning.,Ding, Jin-Wang.,Peng, You.,Pan, Gang.,...&Luo, Ding-Cun.(2022).Identification of Long Noncoding RNAs Associated With the Clinicopathological Features of Papillary Thyroid Carcinoma Complicated With Hashimoto's Thyroiditis.FRONTIERS IN ONCOLOGY,12.
MLA	Zhang, Yu,et al."Identification of Long Noncoding RNAs Associated With the Clinicopathological Features of Papillary Thyroid Carcinoma Complicated With Hashimoto's Thyroiditis".FRONTIERS IN ONCOLOGY 12(2022).