Off-Chip Vertical Step Emulsification Droplets Preparation Device Applied for Droplet Digital PCR

doi:10.1002/admi.202001074

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	Off-Chip Vertical Step Emulsification Droplets Preparation Device Applied for Droplet Digital PCR
	B. Shi,D. Wu,Y. Y. Jiang,J. R. An and W. M. Wu
刊名	Advanced Materials Interfaces
	2020
卷号	7 期号:22 页码:7
ISSN号	2196-7350
DOI	10.1002/admi.202001074
英文摘要	High efficient and stable droplets preparation is an essential technology in digital PCR (dPCR) and other analytical fields. In this paper, a microfluidic device called vertical step emulsification (VSE) device to produce emulsion droplets rapidly off-chip is developed. VSE device prepares droplets by perfluoroalkoxy (PFA) capillary and microcentrifuge tube, when the continuous water phase in PFA capillary jets into the static oil phase in the microcentrifuge tube at a certain speed, the continuous water phase is emulsified into stable droplets due to the sudden change of surface tension. In order to control the droplet size accurately, a general screw mechanism to control the gap between the PFA capillary outlet and the bottom of the inner wall of microcentrifuge tube is used. Meanwhile, dPCR tests with high dynamic range throughput droplets from VSE device are performed. The results show that the off-chip droplet preparation device developed by the authors not only gets rid of the complicated control structure, has the advantages of low cost, easy operation, and user-friendly, but also provides a sustainable development scheme for the preparation of high-throughput and stable droplets in a short time.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.ciomp.ac.cn/handle/181722/64741]
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	B. Shi,D. Wu,Y. Y. Jiang,J. R. An and W. M. Wu. Off-Chip Vertical Step Emulsification Droplets Preparation Device Applied for Droplet Digital PCR[J]. Advanced Materials Interfaces,2020,7(22):7.
APA	B. Shi,D. Wu,Y. Y. Jiang,J. R. An and W. M. Wu.(2020).Off-Chip Vertical Step Emulsification Droplets Preparation Device Applied for Droplet Digital PCR.Advanced Materials Interfaces,7(22),7.
MLA	B. Shi,D. Wu,Y. Y. Jiang,J. R. An and W. M. Wu."Off-Chip Vertical Step Emulsification Droplets Preparation Device Applied for Droplet Digital PCR".Advanced Materials Interfaces 7.22(2020):7.