A digital PCR system based on the thermal cycled chip with multi helix winding capillary

doi:10.1038/s41598-020-74711-8

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	A digital PCR system based on the thermal cycled chip with multi helix winding capillary
	B. Li,Y. M. Li,Y. Y. Jiang,A. Manz and W. M. Wu
刊名	Scientific Reports
	2020
卷号	10 期号:1 页码:8
ISSN号	2045-2322
DOI	10.1038/s41598-020-74711-8
英文摘要	This paper presents a digital PCR system based on a novel thermal cycled chip, which wraps microchannels on a trapezoidal structure made of polydimethylsiloxane (PDMS) in a multi-helix manner for the first time. It is found that compared to the single helix chip commonly used in previous reports, this kind of novel multi-helix chip can make the surface temperature in the renaturation zone more uniform, and even in the case of rapid fluid flow, it can improve the efficiency of the polymerase chain reaction. What's more, the winding method of multi helix (such as double helix, six helix and eight helix) can obtain better temperature uniformity than the winding of odd helix (such as single helix and three helix). As a proof of concept, the temperature-optimized double-helical chip structure is applied to continuous-flow digital PCR and there is no need to add any surfactant to both the oil phase and reagent. In addition, we successfully analyzed the fluorescence signal of continuous-flow digital PCR by using CMOS camera. Finally, this method is applied for the absolute quantification of the clinical serum sample infected by HBV. The accuracy of the test results has been confirmed by commercial instruments.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.ciomp.ac.cn/handle/181722/64453]
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	B. Li,Y. M. Li,Y. Y. Jiang,A. Manz and W. M. Wu. A digital PCR system based on the thermal cycled chip with multi helix winding capillary[J]. Scientific Reports,2020,10(1):8.
APA	B. Li,Y. M. Li,Y. Y. Jiang,A. Manz and W. M. Wu.(2020).A digital PCR system based on the thermal cycled chip with multi helix winding capillary.Scientific Reports,10(1),8.
MLA	B. Li,Y. M. Li,Y. Y. Jiang,A. Manz and W. M. Wu."A digital PCR system based on the thermal cycled chip with multi helix winding capillary".Scientific Reports 10.1(2020):8.