Real-time red blood cell counting and osmolarity analysis using a photoacoustic-based microfluidic system | |
Zhao WX(赵文秀)1,2,4; Yu HB(于海波)2,4; Wen YD(文扬东)1,2,4; Luo H(罗昊)1,2,4; Jia BL(贾伯良)3; Wang XD(王晓朵)2,4; Liu LQ(刘连庆)2,4; Li WJ(李文荣)2,3,4 | |
刊名 | LAB ON A CHIP |
2021 | |
卷号 | 21期号:13页码:2586-2593 |
ISSN号 | 1473-0197 |
产权排序 | 1 |
英文摘要 | Counting the number of red blood cells (RBCs) in blood samples is a common clinical diagnostic procedure, but conventional methods are unable to provide the size and other physical properties of RBCs at the same time. In this work, we explore photoacoustic (PA) detection as a rapid label-free and noninvasive analysis technique that can potentially be used for single RBC characterization based on their photoabsorption properties. We have demonstrated an on-chip PA flow cytometry system using a simple microfluidic chip combined with a PA imaging system to count and characterize up to similar to 60 RBCs per second. Compared with existing microfluidic-based RBC analysis methods, which typically use camera-captured image sequences to characterize cell morphology and deformation, the PA method discussed here requires only the processing of one-dimensional time-series data instead of two- or three-dimensional time-series data acquired by computer vision methods. Therefore, the PA method will have significantly lower computational requirements when large numbers of RBCs are to be analyzed. Moreover, we have demonstrated that the PA signals of RBCs flowing in a microfluidic device could be directly used to acquire the osmolarity conditions (in the range of 124 to 497 mOsm L-1) of the medium surrounding the RBCs. This finding suggests a potential extension of applicability to blood tests via PA-based biomedical detection. |
资助项目 | National Natural Science Foundation of China[61925307] ; National Natural Science Foundation of China[61727811] ; National Natural Science Foundation of China[U1908215] ; National Natural Science Foundation of China[61973298] ; External Cooperation Program of the Chinese Academy of Sciences[173321KYSB20170015] ; CAS Interdisciplinary Innovation Team[JCTD-2019-09] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[Y201943] ; Hong Kong Research Grants Council[9042639] ; Hong Kong Research Grants Council[907002] ; Hong Kong Research Grants Council[JLFS/E-104/18] |
WOS关键词 | ERYTHROCYTES |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation |
语种 | 英语 |
WOS记录号 | WOS:000651770500001 |
资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [61925307, 61727811, U1908215, 61973298] ; External Cooperation Program of the Chinese Academy of SciencesChinese Academy of Sciences [173321KYSB20170015] ; CAS Interdisciplinary Innovation Team [JCTD-2019-09] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences [Y201943] ; Hong Kong Research Grants CouncilHong Kong Research Grants Council [9042639, 907002, JLFS/E-104/18] |
内容类型 | 期刊论文 |
源URL | [http://ir.sia.cn/handle/173321/28905] |
专题 | 沈阳自动化研究所_机器人学研究室 |
通讯作者 | Liu LQ(刘连庆); Li WJ(李文荣) |
作者单位 | 1.University of Chinese Academy of Sciences, Beijing 100049, China 2.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China 3.Department of Mechanical Engineering, City University of Hong Kong, Hong Kong, SAR, China 4.Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang 110016, China |
推荐引用方式 GB/T 7714 | Zhao WX,Yu HB,Wen YD,et al. Real-time red blood cell counting and osmolarity analysis using a photoacoustic-based microfluidic system[J]. LAB ON A CHIP,2021,21(13):2586-2593. |
APA | Zhao WX.,Yu HB.,Wen YD.,Luo H.,Jia BL.,...&Li WJ.(2021).Real-time red blood cell counting and osmolarity analysis using a photoacoustic-based microfluidic system.LAB ON A CHIP,21(13),2586-2593. |
MLA | Zhao WX,et al."Real-time red blood cell counting and osmolarity analysis using a photoacoustic-based microfluidic system".LAB ON A CHIP 21.13(2021):2586-2593. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论