Atomic Force Microscopy as a Powerful Multifunctional Tool for Probing the Behaviors of Single Proteins | |
Li M(李密)2,3,4; Wang YC(王越超)2,3,4; Liu LQ(刘连庆)2,3,4 | |
刊名 | IEEE TRANSACTIONS ON NANOBIOSCIENCE |
2020 | |
卷号 | 19期号:1页码:78-99 |
关键词 | Atomic force microscopy (AFM) single-protein analysis morphological imaging single-molecule force recognition mechanical unfolding high-speed AFM |
ISSN号 | 1536-1241 |
产权排序 | 1 |
英文摘要 | Proteins play a crucial role in regulating life activities and therefore investigating the behaviors of proteins is of critical significance for understanding the underlying mechanisms guiding the physiological and pathological processes of living organisms. Traditional molecular biochemical methods for protein assays are based on ensemble measurements which reflect the averaged behaviors of the whole molecular populations, veiling the rare activities of small molecular subpopulations. Achievements obtained in the past decades have proved that atomic force microscopy (AFM) is a powerful multifunctional tool for characterizing the behaviors of single proteins at work in aqueous conditions with unprecedented spatiotemporal resolution, which provides novel insights into nanoscopic molecular mechanisms and contributes much to the communities of protein biology. In this article, we review the recent advances in AFM-based single-protein analysis from several facets (including morphological imaging, single-molecule force recognition, mechanical unfolding pathways, and high-speed AFM molecular detection), and provide perspectives for future progression. |
资助项目 | National Natural Science Foundation of China[61922081] ; National Natural Science Foundation of China[61873258] ; National Natural Science Foundation of China[U1613220] ; Research Program of Frontier Sciences CAS[ZDBS-LY-JSC043] ; Youth Innovation Promotion Association CAS[2017243] |
WOS关键词 | MEMBRANE-PROTEINS ; SUBNANOMETER RESOLUTION ; SIMULTANEOUS TOPOGRAPHY ; MOLECULAR-INTERACTIONS ; CONFORMATIONAL-CHANGES ; UNFOLDING PATHWAYS ; LATERAL DIFFUSION ; PLASMA-MEMBRANE ; NATIVE PROTEINS ; CELL-MEMBRANES |
WOS研究方向 | Biochemistry & Molecular Biology ; Science & Technology - Other Topics |
语种 | 英语 |
WOS记录号 | WOS:000506601200009 |
资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China [61922081, 61873258, U1613220] ; Research Program of Frontier Sciences CAS [ZDBS-LY-JSC043] ; Youth Innovation Promotion Association CAS [2017243] |
内容类型 | 期刊论文 |
源URL | [http://ir.sia.cn/handle/173321/26220] |
专题 | 沈阳自动化研究所_机器人学研究室 |
通讯作者 | Li M(李密) |
作者单位 | 1.Department of Industrial and Manufacturing Systems Engineering, University of Hong Kong, Hong Kong 2.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China 3.Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang 110016, China 4.University of Chinese Academy of Sciences, Beijing 100049, China |
推荐引用方式 GB/T 7714 | Li M,Wang YC,Liu LQ. Atomic Force Microscopy as a Powerful Multifunctional Tool for Probing the Behaviors of Single Proteins[J]. IEEE TRANSACTIONS ON NANOBIOSCIENCE,2020,19(1):78-99. |
APA | Li M,Wang YC,&Liu LQ.(2020).Atomic Force Microscopy as a Powerful Multifunctional Tool for Probing the Behaviors of Single Proteins.IEEE TRANSACTIONS ON NANOBIOSCIENCE,19(1),78-99. |
MLA | Li M,et al."Atomic Force Microscopy as a Powerful Multifunctional Tool for Probing the Behaviors of Single Proteins".IEEE TRANSACTIONS ON NANOBIOSCIENCE 19.1(2020):78-99. |
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