A ratiometric fluorescent probe based on carbon dots assembly for intracellular lysosomal polarity imaging with wide range response

doi:10.1016/j.cclet.2021.08.076

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	A ratiometric fluorescent probe based on carbon dots assembly for intracellular lysosomal polarity imaging with wide range response
	Zou, Guangyue 2; Chen, Shuai 2; Liu, Nazhen 1; Yu, Yongliang 2
刊名	CHINESE CHEMICAL LETTERS
	2022-02-01
卷号	33 期号:2 页码:778-782
关键词	Carbon dots Polarity Lysosome Ratiometric sensing Wide linear range
ISSN号	1001-8417
DOI	10.1016/j.cclet.2021.08.076
通讯作者	Liu, Nazhen(liunazhen@qdio.ac.cn) ; Yu, Yongliang(yuyl@mail.neu.edu.cn)
英文摘要	Lysosomal polarity is considered a key indicator of lysosomal function due to its significant impact on membrane fluidity and enzymatic reactions in lysosomes. Monitoring lysosomal polarity can gain insight into the related physiological and pathological processes and develop new diagnostic methods. However, current fluorescent probes with lysosomal polarity response suffer from narrow linear range, photo-bleaching and complicated preparation. Herein, a ratiometric fluorescent probe (r-bCDs) for intracellular lysosomal polarity imaging is designed and constructed by amide bond assembly of polarity-sensitive red fluorescent carbon dots (rCDs) and referenced blue fluorescent carbon dots (bCDs). r-bCDs show a much wider linear range of polarity response (orientation polarizability Delta f from 0.020 to 0.315) than other probes, and the interference of uneven distribution and instrument factors can be effectively eliminated by ratiometric fluorescent sensing. Imaging of intracellular lysosomal polarity with r-bCDs is implemented to observe the polarity variation caused by the change of cell state and the difference between cancer cells and normal cells. This work provides a promising tool for studying the related physiological and pathological processes and developing new diagnostic methods. (C) 2021 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
资助项目	National Natural Science Foundation of China[21922402] ; National Natural Science Foundation of China[21874017] ; National Natural Science Foundation of China[21727811] ; Fundamental Research Funds for the Central Universities[N2005027]
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000760891400023
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/178182]
专题	海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者	Liu, Nazhen; Yu, Yongliang
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Northeastern Univ, Coll Sci, Res Ctr Analyt Sci, Dept Chem, Shenyang 110819, Peoples R China
推荐引用方式 GB/T 7714	Zou, Guangyue,Chen, Shuai,Liu, Nazhen,et al. A ratiometric fluorescent probe based on carbon dots assembly for intracellular lysosomal polarity imaging with wide range response[J]. CHINESE CHEMICAL LETTERS,2022,33(2):778-782.
APA	Zou, Guangyue,Chen, Shuai,Liu, Nazhen,&Yu, Yongliang.(2022).A ratiometric fluorescent probe based on carbon dots assembly for intracellular lysosomal polarity imaging with wide range response.CHINESE CHEMICAL LETTERS,33(2),778-782.
MLA	Zou, Guangyue,et al."A ratiometric fluorescent probe based on carbon dots assembly for intracellular lysosomal polarity imaging with wide range response".CHINESE CHEMICAL LETTERS 33.2(2022):778-782.