Detection of bloom-forming dinoflagellates Karenia mikimotoi and Prorocentrum donghaiense using qPCR assays

doi:10.1007/s10811-022-02698-y

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Detection of bloom-forming dinoflagellates Karenia mikimotoi and Prorocentrum donghaiense using qPCR assays
	Xiao-Kun Hu 6,7; Zhuo-Ru Lin 6,7; Qing-Chun Zhang 4,5,7; Fan-Zhou Kong 4,5,7; Jing-Yi Cen 3; Yu-Lan Zeng 1,2; Ren-Cheng Yu 4,5,6,7
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2022-02-05
页码	14
关键词	Harmful algal bloom Karenia mikimotoi Prorocentrum donghaiense Prorocentrum obtusidens qPCR East China Sea
ISSN号	0921-8971
DOI	10.1007/s10811-022-02698-y
通讯作者	Ren-Cheng Yu(rcyu@qdio.ac.cn)
英文摘要	The dinoflagellates Karenia mikimotoi and Prorocentrum donghaiense are both important causative species of harmful algal blooms (HABs) in the East China Sea. The ichthyotoxic K. mikimotoi, which occasionally leads to large-scale HABs in the East China Sea, is difficult to be discriminated from other morphologically similar species in the family Kareniaceae by light microscope observation. To improve the accuracy and efficiency in detection of K. mikimotoi, a real-time quantitative PCR (qPCR) assay was developed in this study. The qPCR assay has high specificity and sensitivity, which allows the detection of K. mikimotoi at the lower detection limit of one cell. The qPCR assay target K. mikimotoi, together with another qPCR assay previously developed for P. donghaiense, was applied to study a bloom of dinoflagellates in the coastal waters of Fujian province from April 25 to June 11 in 2019. Cells of K. mikimotoi were detected in about half of the samples, and the maximum abundance was lower than 30 cells L-1. Abundance of P. donghaiense cells (maximum abundance above 10(6) cells L-1) were determined using both qPCR assay and light microscope cell counting, and the results of the two methods were consistent with each other. The qPCR assays of the blooming dinoflagellates offer reliable and accurate approaches for the detection of HABs species.
资助项目	National Key R&D Program of China[2017YFC1404304] ; Strategic Priority Research Program of Chinese Academy of Sciences (CAS)[XDA19060203] ; CAS-CSIRO BAU project of CAS[133137KYSB20180141] ; Zhejiang Provincial Natural Science Foundation of China[LY19D060007]
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000751579800002
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/177960]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Ren-Cheng Yu
作者单位	1.Zhejiang Univ, Coll Agr & Biotechnol, Hangzhou 310058, Peoples R China 2.Minist Nat Resources, Key Lab Marine Ecosyst Dynam, Inst Oceanog 2, Hangzhou 310012, Peoples R China 3.Jinan Univ, Coll Life Sci & Technol, Guangzhou 510632, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 5.Pilot Natl Lab Marine Sci & Technol Qingdao, Key Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Xiao-Kun Hu,Zhuo-Ru Lin,Qing-Chun Zhang,et al. Detection of bloom-forming dinoflagellates Karenia mikimotoi and Prorocentrum donghaiense using qPCR assays[J]. JOURNAL OF APPLIED PHYCOLOGY,2022:14.
APA	Xiao-Kun Hu.,Zhuo-Ru Lin.,Qing-Chun Zhang.,Fan-Zhou Kong.,Jing-Yi Cen.,...&Ren-Cheng Yu.(2022).Detection of bloom-forming dinoflagellates Karenia mikimotoi and Prorocentrum donghaiense using qPCR assays.JOURNAL OF APPLIED PHYCOLOGY,14.
MLA	Xiao-Kun Hu,et al."Detection of bloom-forming dinoflagellates Karenia mikimotoi and Prorocentrum donghaiense using qPCR assays".JOURNAL OF APPLIED PHYCOLOGY (2022):14.