Haloalkaliphilic denitrifiers-dependent sulfate-reducing bacteria thrive in nitrate-enriched environments

doi:10.1016/j.watres.2021.117354

	Haloalkaliphilic denitrifiers-dependent sulfate-reducing bacteria thrive in nitrate-enriched environments
	Zhou, Jiemin 1; Xing, Jianmin 2
刊名	WATER RESEARCH
	2021-08-01
卷号	201 页码:8
关键词	Sulfate-reducing bacteria Denitrifying bacteria Metatranscriptomic analysis Nitrate inhibition Enriched biomass
ISSN号	0043-1354
DOI	10.1016/j.watres.2021.117354
英文摘要	As bridge in global cycles of carbon, nitrogen, and sulfur, sulfate-reducing bacteria (SRB) play more and more important role under various environments, especially the saline-alkali environments with significant increase in area caused by human activities. Sulfate reduction can be inhibited by environmental nitrate. However, how SRB cope with environmental nitrate stress in these extreme environments still remain unclear. Here, after a longterm enrichment of sediment from saline-alkali Qinghai Lake of China using anaerobic filter reactors, nitrate was added to evaluate the response of SRB. With the increase in nitrate concentrations, the inhibition on sulfate reduction was gradually observed. Interestingly, extension of hydraulic retention time can relieve the inhibition caused by high nitrate concentration. Mass balance analysis showed that nitrate reduction is prior to sulfate reduction. Further metatranscriptomic analysis shows that, genes of nitrite reductase (periplasmic cytochrome c nitrite reductase gene) and energy metabolisms (lactate dehydrogenase, formate dehydrogenase, pyruvate: ferredoxin-oxidoreductase, and fumarate reductase genes) in SRB was down-regulated, challenging the longheld opinion that up-regulation of these genes can relieve the nitrate inhibition. Most importantly, the nitrate addition activated the denitrification pathway in denitrifying bacteria (DB) via significantly up-regulating the expression of the corresponding genes (nitrite reductase, nitric oxide reductase c subunit, nitric oxide reductase activation protein and nitrous oxide reductase genes), quickly reducing the environmental nitrate and relieving the nitrate inhibition on SRB. Our findings unravel that in response to environmental nitrate stress, haloalkaliphilic SRB show dependency on DB, and expand our knowledge of microbial relationship during sulfur and nitrogen cycles.
资助项目	Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences[19Z02KLDWST]
WOS关键词	DESULFOVIBRIO-VULGARIS HILDENBOROUGH ; GENE-EXPRESSION ANALYSIS ; ELECTRON-DONORS ; RNA-SEQ ; PERFORMANCE ; INHIBITION ; STRESS ; TRANSCRIPTOMES ; CONSEQUENCES ; COMMUNITIES
WOS研究方向	Engineering ; Environmental Sciences & Ecology ; Water Resources
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000681702200007
资助机构	Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/49608]
专题	中国科学院过程工程研究所
通讯作者	Zhou, Jiemin
作者单位	1.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Key Lab Drinking Water Sci & Technol, Beijing 100085, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Jiemin,Xing, Jianmin. Haloalkaliphilic denitrifiers-dependent sulfate-reducing bacteria thrive in nitrate-enriched environments[J]. WATER RESEARCH,2021,201:8.
APA	Zhou, Jiemin,&Xing, Jianmin.(2021).Haloalkaliphilic denitrifiers-dependent sulfate-reducing bacteria thrive in nitrate-enriched environments.WATER RESEARCH,201,8.
MLA	Zhou, Jiemin,et al."Haloalkaliphilic denitrifiers-dependent sulfate-reducing bacteria thrive in nitrate-enriched environments".WATER RESEARCH 201(2021):8.