Coupling the fermentation and membrane separation process for polyamides monomer cadaverine production from feedstock lysine

doi:10.1002/elsc.202000099

	Coupling the fermentation and membrane separation process for polyamides monomer cadaverine production from feedstock lysine
	Luo, Ruoshi 2,3; Qin, Zhao 2; Zhou, Dan 2; Wang, Dan 2,3; Hu, Ge 2; Su, Zhiguo 1; Zhang, Suojiang 1
刊名	ENGINEERING IN LIFE SCIENCES
	2021-06-10
页码	7
关键词	batch fermentation cadaverine lysine decarboxylases membrane separation polyamides monomer
ISSN号	1618-0240
DOI	10.1002/elsc.202000099
英文摘要	Nylon is a polyamide material with excellent performance used widely in the aviation and automobile industries, and other fields. Nylon monomers such as hexamethylene diamine and other monomers are in huge demand. Therefore, in order to expand the methods of nylon production, we tried to develop alternative bio-manufacturing processes which would make a positive contribution to the nylon industry. In this study, the engineered E. coli-overexpressing Lysine decarboxylases (LDCs) were used for the bioconversion of l-lysine to cadaverine. An integrated fermentation and microfiltration (MF) process for high-level cadaverine production by E. coli was established. Concentration was increased from 87 to 263.6 g/L cadaverine after six batch coupling with a productivity of 3.65 g/L-h. The cadaverine concentration was also increased significantly from 0.43 g cadaverine/g l-lysine to 0.88 g cadaverine/g l-lysine by repeated batch fermentation. These experimental results indicate that coupling the fermentation and membrane separation process could benefit the continuous production of cadaverine at high levels.
资助项目	National Natural Science Foundation of China[21978027] ; Fundamental Research Funds for the Central Universities[2020CDCGJ020] ; Fundamental Research Funds for the Central Universities[2020CDCGHG068] ; Scientific Research Foundation of State Key Lab. of Coal Mine Disaster Dynamics and Control[2011DA105287-ZR202002] ; Key Projects of Technological Innovation and Application Development of Chongqing, China[cstc 2019jscx-fxydX0024]
WOS关键词	LACTIC-ACID PRODUCTION ; ESCHERICHIA-COLI ; SUCCINIC ACID
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	WILEY
WOS记录号	WOS:000659724800001
资助机构	National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; Scientific Research Foundation of State Key Lab. of Coal Mine Disaster Dynamics and Control ; Key Projects of Technological Innovation and Application Development of Chongqing, China
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/49072]
专题	中国科学院过程工程研究所
通讯作者	Wang, Dan
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Beijing, Peoples R China 2.Chongqing Univ, Dept Chem Engn, Sch Chem & Chem Engn, Chongqing 401331, Peoples R China 3.Chongqing Univ, State Key Lab Coal Mine Disaster Dynam & Control, Chongqing 401331, Peoples R China
推荐引用方式 GB/T 7714	Luo, Ruoshi,Qin, Zhao,Zhou, Dan,et al. Coupling the fermentation and membrane separation process for polyamides monomer cadaverine production from feedstock lysine[J]. ENGINEERING IN LIFE SCIENCES,2021:7.
APA	Luo, Ruoshi.,Qin, Zhao.,Zhou, Dan.,Wang, Dan.,Hu, Ge.,...&Zhang, Suojiang.(2021).Coupling the fermentation and membrane separation process for polyamides monomer cadaverine production from feedstock lysine.ENGINEERING IN LIFE SCIENCES,7.
MLA	Luo, Ruoshi,et al."Coupling the fermentation and membrane separation process for polyamides monomer cadaverine production from feedstock lysine".ENGINEERING IN LIFE SCIENCES (2021):7.